Anti-Hec1 Antibody (1) recommended for detection of Hec1 of mouse, rat, human and canine origin by WB, IP and IF

Anti-Hec1 Antibody (1): sc-135934

Anti-Hec1 Antibody (1) is rated 5.0 out of 5 by 3.
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Datasheets

    • Hec1 Antibody (1) is a mouse monoclonal IgG1, cited in 3 publications, provided at 50 µg/500 µl
    • raised against amino acids 495-608 of Hec1 of human origin
    • recommended for detection of Hec1 of mouse, rat, human and canine origin by WB, IP and IF
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Hec1 Antibody (1). This work is in progress.
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I am using Hec1 (1): sc-135934 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from gave a single band Ran cell lysate on a 10 percent gel and transfered to PVDF using 10% Methanol Transfer buffer in a semi dry apparatus. Antibody gave a single band at approximately the expected molecular weight. Hec1 knockdown by siRNA was reflected in the band intensity by western.
Date published: 2017-02-15
Rated 5 out of 5 by from Excellent immunofluorescence cytoplasmic Excellent immunofluorescence cytoplasmic staining in BC3H1 cells. -SCBT QC
Date published: 2015-02-25
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