Headpin Antibody (H-7): sc-393306
- Headpin Antibody (H-7) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 255-282 within an internal region of Headpin of rat origin
- Anti-Headpin Antibody (H-7) is recommended for detection of Headpin of mouse and rat origin by WB, IP, IF and ELISA
- Anti-Headpin Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Headpin Antibody (H-7) for WB applications. These reagents are now offered in bundles with Headpin Antibody (H-7) (see ordering information below).
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SEE ALSO...
Headpin Antibody (H-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects Headpin in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Headpin antibody (H-7) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Headpin, also known as hurpin or serpinB13, is a skin-specific serine proteinase inhibitor that plays a crucial role in regulating proteolytic activity in the skin, which is vital for maintaining skin integrity and homeostasis. Headpin (H-7) antibody targets a protein that is particularly abundant in the human keratinocyte cell line HaCaT and is found in lesional keratinocytes from psoriatic skin, highlighting its importance in skin health and disease. Notably, downregulation of Headpin has been observed in squamous cell carcinoma of the oral cavity and in squamous cell carcinoma cell lines from the head and neck, suggesting its potential role as a biomarker for skin cancer progression and a target for therapeutic intervention.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Headpin Antibody (H-7) References:
- Cloning and characterization of hurpin (protease inhibitor 13): A new skin-specific, UV-repressible serine proteinase inhibitor of the ovalbumin serpin family. | Abts, HF., et al. 1999. J Mol Biol. 293: 29-39. PMID: 10512713
- Identification and cDNA cloning of headpin, a novel differentially expressed serpin that maps to chromosome 18q. | Spring, P., et al. 1999. Biochem Biophys Res Commun. 264: 299-304. PMID: 10527881
- Genomic cloning, mapping, structure and promoter analysis of HEADPIN, a serpin which is down-regulated in head and neck cancer cells. | Nakashima, T., et al. 2000. Biochim Biophys Acta. 1492: 441-6. PMID: 11004515
- Inhibition of the cysteine proteinases cathepsins K and L by the serpin headpin (SERPINB13): a kinetic analysis. | Jayakumar, A., et al. 2003. Arch Biochem Biophys. 409: 367-74. PMID: 12504904
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Headpin Antibody (H-7) | sc-393306 | 200 µg/ml | $322.00 | |||
Headpin Antibody (H-7): m-IgG Fc BP-HRP Bundle | sc-530424 | 200 µg Ab; 10 µg BP | $361.00 | |||
Headpin Antibody (H-7): m-IgGκ BP-HRP Bundle | sc-523896 | 200 µg Ab, 40 µg BP | $361.00 | |||
Headpin Antibody (H-7) AC | sc-393306 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Headpin Antibody (H-7) HRP | sc-393306 HRP | 200 µg/ml | $322.00 | |||
Headpin Antibody (H-7) FITC | sc-393306 FITC | 200 µg/ml | $336.00 | |||
Headpin Antibody (H-7) PE | sc-393306 PE | 200 µg/ml | $349.00 | |||
Headpin Antibody (H-7) Alexa Fluor® 488 | sc-393306 AF488 | 200 µg/ml | $364.00 | |||
Headpin Antibody (H-7) Alexa Fluor® 546 | sc-393306 AF546 | 200 µg/ml | $364.00 | |||
Headpin Antibody (H-7) Alexa Fluor® 594 | sc-393306 AF594 | 200 µg/ml | $364.00 | |||
Headpin Antibody (H-7) Alexa Fluor® 647 | sc-393306 AF647 | 200 µg/ml | $364.00 | |||
Headpin Antibody (H-7) Alexa Fluor® 680 | sc-393306 AF680 | 200 µg/ml | $364.00 | |||
Headpin Antibody (H-7) Alexa Fluor® 790 | sc-393306 AF790 | 200 µg/ml | $364.00 | |||
Headpin (H-7) Neutralizing Peptide | sc-393306 P | 100 µg/0.5 ml | $69.00 |