HDEL Antibody (2E7): sc-53472
- HDEL Antibody (2E7) is a mouse monoclonal IgG2b κ HDEL antibody, cited in 26 publications, provided at 200 µg/ml
- raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast BiP (also designated GRP 78)
- HDEL Antibody (2E7) is recommended for detection of microsomal membrane proteins of mouse, rat, human, Saccharomyces cerevisiae, Drosophila melanogaster and Arabidopsis thaliana origin by WB, IP, IF and IHC(P)
- Anti-HDEL Antibody (2E7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for HDEL Antibody (2E7) for WB and IHC(P) applications. This reagent is now offered in a bundle with HDEL Antibody (2E7) (see ordering information below).
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HDEL Antibody (2E7) is a mouse monoclonal IgG2b kappa light chain antibody that detects the HDEL protein of mouse, rat, human, and various model organisms including Saccharomyces cerevisiae, Drosophila melanogaster, and Arabidopsis thaliana by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-HDEL antibody (2E7) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. HDEL protein, particularly the GRP 78 variant, plays a crucial role as a molecular chaperone within the endoplasmic reticulum (ER), where HDEL assists in the proper folding and translocation of nascent polypeptide chains. This function is vital for maintaining cellular homeostasis and ensuring that proteins are correctly processed before secretion or transport to final destinations. HDEL (2E7) monoclonal antibody specifically recognizes the C-terminal HDEL sequence, a retention signal essential for protein localization within the ER, and effectively stains HDEL proteins in various plant species, including maize, onion, barnyard grass, beet, cotton, mung bean, sorghum, and wheat. HDEL (2E7) antibody enables researchers to gain insights into protein folding and retention dynamics in the ER, which is critical for understanding various cellular processes and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HDEL Antibody (2E7) References:
- Prevention of protein denaturation under heat stress by the chaperonin Hsp60. | Martin, J., et al. 1992. Science. 258: 995-8. PMID: 1359644
- Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. | Napier, RM., et al. 1992. J Cell Sci. 102 (Pt 2): 261-71. PMID: 1383243
- Effects of low culture temperature on the induction of hsp70 mRNA and the accumulation of hsp70 and hsp105 in mouse FM3A cells. | Hatayama, T., et al. 1992. J Biochem. 111: 484-90. PMID: 1618738
- Cloning of rat grp75, an hsp70-family member, and its expression in normal and ischemic brain. | Massa, SM., et al. 1995. J Neurosci Res. 40: 807-19. PMID: 7629893
- Protein-mediated protein maturation in eukaryotes. | Haas, IG. 1995. FEBS Lett. 369: 72-5. PMID: 7641888
- Can Hsp70 proteins act as force-generating motors? | Glick, BS. 1995. Cell. 80: 11-4. PMID: 7813006
- Autophosphorylation of grp94 (endoplasmin). | Csermely, P., et al. 1995. J Biol Chem. 270: 6381-8. PMID: 7890776
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HDEL Antibody (2E7) | sc-53472 | 200 µg/ml | $316.00 | |||
HDEL Antibody (2E7): m-IgG2b BP-HRP Bundle | sc-548656 | 200 µg Ab; 10 µg BP | $354.00 | |||
HDEL Antibody (2E7) AC | sc-53472 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HDEL Antibody (2E7) HRP | sc-53472 HRP | 200 µg/ml | $316.00 | |||
HDEL Antibody (2E7) FITC | sc-53472 FITC | 200 µg/ml | $330.00 | |||
HDEL Antibody (2E7) PE | sc-53472 PE | 200 µg/ml | $343.00 | |||
HDEL Antibody (2E7) Alexa Fluor® 488 | sc-53472 AF488 | 200 µg/ml | $357.00 | |||
HDEL Antibody (2E7) Alexa Fluor® 546 | sc-53472 AF546 | 200 µg/ml | $357.00 | |||
HDEL Antibody (2E7) Alexa Fluor® 594 | sc-53472 AF594 | 200 µg/ml | $357.00 | |||
HDEL Antibody (2E7) Alexa Fluor® 647 | sc-53472 AF647 | 200 µg/ml | $357.00 | |||
HDEL Antibody (2E7) Alexa Fluor® 680 | sc-53472 AF680 | 200 µg/ml | $357.00 | |||
HDEL Antibody (2E7) Alexa Fluor® 790 | sc-53472 AF790 | 200 µg/ml | $357.00 |