HCP1 Antibody (B-4): sc-393460

HCP1 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects HCP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HCP1 Antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HCP1, also known as heme carrier protein 1 or proton-coupled folate transporter (PCFT), is a multi-pass transmembrane protein predominantly expressed in the small intestine, particularly localized to the apical brush border of the duodenum and jejunum. HCP1 plays a crucial role in folate absorption, which is essential for DNA synthesis and repair, making HCP1 vital for cellular function and overall health. HCP1 operates optimally at a low extracellular pH of approximately 5.5, facilitating folate transport independently of sodium ions and membrane potential. HCP1 exhibits a high affinity for folic acid while showing low affinity for PT523 antifolate. Notably, HCP1 undergoes post-translational regulation by iron levels in the duodenum; during iron deficiency, HCP1 localizes to the apical membrane, whereas excess iron causes HCP1 to shift to the cytoplasm. Additionally, sulfasalazine acts as a potent inhibitor of HCP1, and mutations in the HCP1 gene can lead to hereditary folate malabsorption, an autosomal recessive disorder.

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HCP1 Antibody (B-4) References:

1. The intestinal heme transporter revealed.  |  Rouault, TA. 2005. Cell. 122: 649-51. PMID: 16143096
2. Identification of an intestinal heme transporter.  |  Shayeghi, M., et al. 2005. Cell. 122: 789-801. PMID: 16143108
3. Recent advances in mammalian haem transport.  |  Latunde-Dada, GO., et al. 2006. Trends Biochem Sci. 31: 182-8. PMID: 16487711
4. Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption.  |  Qiu, A., et al. 2006. Cell. 127: 917-28. PMID: 17129779
5. Haem carrier protein 1 (HCP1): Expression and functional studies in cultured cells.  |  Latunde-Dada, GO., et al. 2006. FEBS Lett. 580: 6865-70. PMID: 17156779
6. Heme carrier protein 1 (HCP1) expression and functional analysis in the retina and retinal pigment epithelium.  |  Sharma, S., et al. 2007. Exp Cell Res. 313: 1251-9. PMID: 17335806
7. The spectrum of mutations in the PCFT gene, coding for an intestinal folate transporter, that are the basis for hereditary folate malabsorption.  |  Zhao, R., et al. 2007. Blood. 110: 1147-52. PMID: 17446347
8. Functional characterization of human proton-coupled folate transporter/heme carrier protein 1 heterologously expressed in mammalian cells as a folate transporter.  |  Nakai, Y., et al. 2007. J Pharmacol Exp Ther. 322: 469-76. PMID: 17475902
9. Rodent intestinal folate transporters (SLC46A1): secondary structure, functional properties, and response to dietary folate restriction.  |  Qiu, A., et al. 2007. Am J Physiol Cell Physiol. 293: C1669-78. PMID: 17898134

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HCP1 Antibody (B-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-HCP1 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HCP1 Antibody (B-4) Specifications

Alternate Product Name: HCP1

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 432-459

Epitope Target: HCP1

Epitope Species: human

Product Reactivity/Detection: HCP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for HCP1 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin