Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Hck CRISPR/Cas9 KO Plasmid (m): sc-420810
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hck CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hck genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hck Antibody (G-4): sc-166463
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hck CRISPR/Cas9 KO Plasmid (m) | sc-420810 | 20 µg | $397.00 |
Overview
Mouse Hck encodes hematopoietic cell kinase, a Src family non-receptor tyrosine kinase enriched in myeloid lineages that couples immunoreceptor and integrin signals to downstream phosphorylation networks. Hck participates in Fc receptor and Toll-like receptor–linked pathways that coordinate cytoskeletal remodeling, phagocytosis, degranulation, and inflammatory mediator production, converging on nodes such as PI3K/AKT, MAPK, and NF-κB. Altered Hck activity has been associated with dysregulated innate immune signaling and aberrant myeloid cell behavior in inflammatory conditions and leukemia models. As a proximal signaling kinase, Hck is widely used to interrogate mechanisms controlling macrophage and neutrophil activation, adhesion, and migration.
Hck CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hck gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hck together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hck open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hck protein expression.
This CRISPR knockout system enables efficient generation of Hck-deficient cell models for investigation of Hck signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hck exon(s) critical for Hck function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hck genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hck CRISPR/Cas9 KO Plasmid (m) and Hck CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hck locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hck HDR Plasmid (m) and Hck HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hck homology arms to support homology-directed repair at defined Hck target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.