Anti-hCAP-D3 Antibody (2B5) is a mouse monoclonal IgG1 (kappa light chain) hCAP-D3 antibody provided at 200 µg/ml
raised against His-tagged recombinant peptide corresponding to amino acids 706-917 of hCAP-D3 of human origin
Anti-hCAP-D3 Antibody (2B5) is recommended for detection of hCAP-D3 of human origin and Ncapd3 of mouse and rat origin of mouse, rat and human origin by WB and IP
Anti-hCAP-D3 Antibody (2B5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of hCAP-D3 (2B5): sc-81597.
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
hCAP-D3 Antibody (2B5) is a high quality monoclonal hCAP-D3 antibody (also designated hCAP-D3 antibody) suitable for the detection of the hCAP-D3 protein of mouse, rat and human origin. hCAP-D3 Antibody (2B5) is available as both the non-conjugated anti-hCAP-D3 antibody form, as well as multiple conjugated forms of anti-hCAP-D3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The condensin complex plays a role in the resolution and segregation of sister chromatids during mitosis and some aspects of mitotic chromosome assembly. Cdc2 phosphorylation of the complex leads to its activation and association with chromosome arms and condensation. Condensin complexes are heteropentamers comprised of two SMC (structural maintenance of chromosomes) subunits and three non-SMC subunits. The SMC family includes SMC1 (also known as SMC1α and SCMB), which associates with SMC3 (also known as hCAP and Bamacan), SMC2 (also known as hCAP-E), which associates with SMC4 (also known as hCAP-C), and SMC5, which associates with SMC6. hCAP-D3 (non-SMC condensin II complex subunit D3), also known as NCAPD3 or KIAA0056, is a 1498 amino acid nuclear protein that contains four HEAT repeats and is a member of the condensin-2 complex.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for hCAP-D3 (2B5): sc-81597 monoclonal antibody?
Asked by: Randy McDonald
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link:
If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
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