CD44 Antibody (OX50): sc-53069

HCAM Antibody (OX50) is a mouse monoclonal IgG1 antibody that detects HCAM in mouse and rat samples through applications such as immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). HCAM, also known as CD44, is a 742 amino acid single-pass type I membrane protein that plays a crucial role in cell adhesion and migration, which are vital processes in embryogenesis, immune response, and tumor metastasis. HCAM functions as a receptor for hyaluronic acid (HA) and interacts with various ligands, including osteopontin (OPN), facilitating both cell-cell and cell-matrix interactions. HCAM′s structure features a Link domain and multiple isoforms generated through alternative splicing, such as CD44R, CDw44, CD44S, CD44H, and CD44E. The CD44H isoform shows high expression in cancer tissues, indicating its potential role in tumor progression and making HCAM a critical target for cancer research. Anti-HCAM antibody (OX50) serves as an essential tool for researchers investigating the dynamics of cell adhesion and migration in various biological contexts, particularly in the study of cancer biology and immune responses.

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CD44 Antibody (OX50) References:

1. Novel hydrazino-carbonyl-amino-methylated polystyrene (HCAM) resin methodology for the synthesis of P1-aldehyde protease inhibitor candidates.  |  Siev, DV. and Semple, JE. 2000. Org Lett. 2: 19-22. PMID: 10814235
2. Fluticasone and salmeterol downregulate in vitro, fibroblast proliferation and ICAM-1 or H-CAM expression.  |  Silvestri, M., et al. 2001. Eur Respir J. 18: 139-45. PMID: 11510784
3. Immunohistochemical detection of the expression of the cell adhesion molecules E-cadherin, desmoglein-2, beta4-integrin, ICAM-1 and HCAM (CD44s) in Warthin's tumour of the parotid gland.  |  Andreadis, D., et al. 2005. Oral Oncol. 41: 799-805. PMID: 16043382
4. H-CAM expression in the human nervous system: evidence for a role in diverse glial interactions.  |  Vogel, H., et al. 1992. J Neurocytol. 21: 363-73. PMID: 1607880
5. Hematopoietic progenitor cell expression of the H-CAM (CD44) homing-associated adhesion molecule.  |  Lewinsohn, DM., et al. 1990. Blood. 75: 589-95. PMID: 1688719
6. The hyaluronate receptor is a member of the CD44 (H-CAM) family of cell surface glycoproteins.  |  Culty, M., et al. 1990. J Cell Biol. 111: 2765-74. PMID: 1703543
7. Expression of beta 1-integrins, H-CAM (CD44) and LECAM-1 in primary gastro-intestinal B-cell lymphomas as compared to the adhesion receptor profile of the gut-associated lymphoid system, tonsil and peripheral lymph node.  |  Möller, P., et al. 1991. Int J Cancer. 49: 846-55. PMID: 1720425
8. Development of new hCaM-Alexa Fluor® biosensors for a wide range of ligands.  |  Velázquez-López, I., et al. 2017. Anal Biochem. 516: 13-22. PMID: 27744023
9. Neoplastic human tissue mast cells express the adhesion molecule CD44/HCAM.  |  Horny, HP., et al. 1996. Virchows Arch. 429: 91-4. PMID: 8917709
10. Flow cytometric detection of CD44 (H-CAM) in hairy cell leukemia.  |  Rutella, S., et al. 1996. Leuk Lymphoma. 21: 497-500. PMID: 9172816

The preferred CD44 antibody is CD44 Antibody (DF1485): sc-7297.