HBXIP Antibody (H-5): sc-373980
- HBXIP Antibody (H-5) is a mouse monoclonal IgG2a κ HBXIP antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-91 representing full length HBXIP of human origin
- HBXIP Antibody (H-5) is recommended for detection of HBXIP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine and porcine
- Anti-HBXIP Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HBXIP Antibody (H-5) for WB and IHC(P) applications. These reagents are now offered in bundles with HBXIP Antibody (H-5) (see ordering information below).
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HBXIP Antibody (H-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects HBXIP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HBXIP antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HBXIP, also known as hepatitis B virus X-interacting protein, plays a crucial role in the life cycle of the hepatitis B virus by negatively regulating the activity of the HBX protein, which is essential for viral replication. HBXIP is evolutionarily conserved and features a leucine zipper motif along with two consensus phosphorylation sites, which are important for function and interactions. HBXIP interacts with survivin, a protein often overexpressed in various human cancers, facilitating survivin′s ability to inhibit pro-caspase-9 activation and thereby promoting cell survival. HBXIP is involved in critical cellular processes such as bipolar spindle formation and the regulation of centrosome dynamics and cytokinesis, potentially through interaction with Dynein light chain. HBXIP overexpression has been linked to increased cell proliferation across multiple cell lines, highlighting significance in both viral pathogenesis and cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HBXIP Antibody (H-5) References:
- HBXIP functions as a cofactor of survivin in apoptosis suppression. | Marusawa, H., et al. 2003. EMBO J. 22: 2729-40. PMID: 12773388
- Hepatitis B virus X protein does not influence essential steps of nucleotide excision repair effected by human liver extracts. | Capovilla, A. and Arbuthnot, P. 2003. Biochem Biophys Res Commun. 312: 806-10. PMID: 14680837
- Upregulation of survivin in G2/M cells and inhibition of caspase 9 activity enhances resistance in staurosporine-induced apoptosis. | Chandele, A., et al. 2004. Neoplasia. 6: 29-40. PMID: 15068669
- An IAP in action: the multiple roles of survivin in differentiation, immunity and malignancy. | Zangemeister-Wittke, U. and Simon, HU. 2004. Cell Cycle. 3: 1121-3. PMID: 15326382
- Hepatitis B virus X protein upregulates survivin expression in hepatoma tissues. | Zhang, X., et al. 2005. J Med Virol. 77: 374-81. PMID: 16173017
- Human ATP-dependent RNA/DNA helicase hSuv3p interacts with the cofactor of survivin HBXIP. | Minczuk, M., et al. 2005. FEBS J. 272: 5008-19. PMID: 16176273
- HBXIP, cellular target of hepatitis B virus oncoprotein, is a regulator of centrosome dynamics and cytokinesis. | Fujii, R., et al. 2006. Cancer Res. 66: 9099-107. PMID: 16982752
- Involvement of hepatitis B X-interacting protein (HBXIP) in proliferation regulation of cells. | Wang, FZ., et al. 2007. Acta Pharmacol Sin. 28: 431-8. PMID: 17303008
- Cloning and characterization of a novel hepatitis B virus x binding protein that inhibits viral replication. | Melegari, M., et al. 1998. J Virol. 72: 1737-43. PMID: 9499022
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HBXIP Antibody (H-5) | sc-373980 | 200 µg/ml | $322.00 | |||
HBXIP Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-529627 | 200 µg Ab; 10 µg BP | $361.00 | |||
HBXIP Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-522579 | 200 µg Ab, 40 µg BP | $361.00 | |||
HBXIP Antibody (H-5): m-IgG2a BP-HRP Bundle | sc-547344 | 200 µg Ab; 10 µg BP | $361.00 | |||
HBXIP Antibody (H-5) AC | sc-373980 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HBXIP Antibody (H-5) HRP | sc-373980 HRP | 200 µg/ml | $322.00 | |||
HBXIP Antibody (H-5) FITC | sc-373980 FITC | 200 µg/ml | $336.00 | |||
HBXIP Antibody (H-5) PE | sc-373980 PE | 200 µg/ml | $349.00 | |||
HBXIP Antibody (H-5) Alexa Fluor® 488 | sc-373980 AF488 | 200 µg/ml | $364.00 | |||
HBXIP Antibody (H-5) Alexa Fluor® 546 | sc-373980 AF546 | 200 µg/ml | $364.00 | |||
HBXIP Antibody (H-5) Alexa Fluor® 594 | sc-373980 AF594 | 200 µg/ml | $364.00 | |||
HBXIP Antibody (H-5) Alexa Fluor® 647 | sc-373980 AF647 | 200 µg/ml | $364.00 | |||
HBXIP Antibody (H-5) Alexa Fluor® 680 | sc-373980 AF680 | 200 µg/ml | $364.00 | |||
HBXIP Antibody (H-5) Alexa Fluor® 790 | sc-373980 AF790 | 200 µg/ml | $364.00 |