Haptoglobin β Antibody (F-8): sc-390962
- Haptoglobin β Antibody (F-8) is a mouse monoclonal IgG1 κ Haptoglobin β antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 181-260 mapping within an internal region of Haptoglobin of human origin
- Haptoglobin beta Antibody (F-8) is recommended for detection of Haptoglobin β and HPR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Haptoglobin beta Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Haptoglobin β Antibody (F-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Haptoglobin β Antibody (F-8) (see ordering information below).
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Haptoglobin β Antibody (F-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Haptoglobin beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Haptoglobin β (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Haptoglobin, a crucial blood plasma protein, plays a vital role in binding free hemoglobin released from erythrocytes, thereby preventing oxidative damage and sequestering iron to inhibit bacterial growth during hemolysis. This protective function is particularly important in inflammatory responses, as elevated Haptoglobin levels can indicate inflammation and assist in the clearance of the Haptoglobin-hemoglobin complex by the reticulo-endothelial system. Structurally, Haptoglobin is composed of two alpha and two beta chains linked by disulfide bridges, resulting from the cleavage of a common precursor protein during synthesis. In humans, Haptoglobin exists in two allelic forms, Haptoglobin 1 (Hp1) and Haptoglobin 2 (Hp2), with Hp2 arising from a partial gene duplication of Hp1. The three known phenotypes—Hp1-1, Hp2-1, and Hp2-2—are associated with various health conditions, including diabetes, cardiovascular diseases, and susceptibility to neurodegenerative disorders like Parkinson′s disease and inflammatory conditions such as Crohn′s disease. Additionally, Haptoglobin is expressed in mammalian hepatocytes and other tissues, including skin, lung, and kidney, making Haptoglobin a valuable biomarker for diagnosing hemolytic anemia and understanding various pathological states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Haptoglobin β Antibody (F-8) References:
- Binding of acellular, native and cross-linked human hemoglobins to haptoglobin: enhanced distribution and clearance in the rat. | Ship, NJ., et al. 2005. Am J Physiol Gastrointest Liver Physiol. 288: G1301-9. PMID: 15890714
- Haptoglobin expression by shed endometrial tissue fragments found in peritoneal fluid. | Sharpe-Timms, KL. 2005. Fertil Steril. 84: 22-30. PMID: 16009149
- The acute phase protein haptoglobin is a mammalian extracellular chaperone with an action similar to clusterin. | Yerbury, JJ., et al. 2005. Biochemistry. 44: 10914-25. PMID: 16086594
- Effect of sucrose on inflammatory markers in overweight humans. | Sørensen, LB., et al. 2005. Am J Clin Nutr. 82: 421-7. PMID: 16087988
- Serum free hemoglobin concentrations in healthy individuals are related to haptoglobin type. | Na, N., et al. 2005. Clin Chem. 51: 1754-5. PMID: 16120964
- Haptoglobin polymorphism predicts 30-day mortality and heart failure in patients with diabetes and acute myocardial infarction. | Suleiman, M., et al. 2005. Diabetes. 54: 2802-6. PMID: 16123372
- Haptoglobin phenotype, sleep-disordered breathing, and the prevalence of cardiovascular disease: the Sleep Heart Health Study. | Levy, AP., et al. 2005. Sleep. 28: 207-13. PMID: 16171245
- C/EBP alpha and C/EBP beta regulate haptoglobin gene expression during rat liver development and the acute-phase response. | Dinić, S., et al. 2005. Mol Biol Rep. 32: 141-7. PMID: 16172914
- Acute-phase related binding ability of p53 for the hormone response element of the haptoglobin gene in adult rats. | Mihailović, M., et al. 2005. Cell Biol Int. 29: 968-70. PMID: 16199185
- Structure of the haptoglobin-haemoglobin complex. | Andersen, CB., et al. 2012. Nature. 489: 456-9. PMID: 22922649
- The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation. | Yang, H., et al. 2017. J Intern Med. 282: 76-93. PMID: 28464519
- Development and validation of a model and nomogram for breast cancer diagnosis based on quantitative analysis of serum disease-specific haptoglobin N-glycosylation. | Li, L., et al. 2024. J Transl Med. 22: 331. PMID: 38575942
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Haptoglobin β Antibody (F-8) | sc-390962 | 200 µg/ml | $322.00 | |||
Haptoglobin β Antibody (F-8): m-IgG Fc BP-HRP Bundle | sc-530316 | 200 µg Ab; 10 µg BP | $361.00 | |||
Haptoglobin β Antibody (F-8): m-IgGκ BP-HRP Bundle | sc-523753 | 200 µg Ab, 40 µg BP | $361.00 | |||
Haptoglobin β Antibody (F-8): m-IgG1 BP-HRP Bundle | sc-543987 | 200 µg Ab; 20 µg BP | $361.00 | |||
Haptoglobin β Antibody (F-8) AC | sc-390962 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Haptoglobin β Antibody (F-8) HRP | sc-390962 HRP | 200 µg/ml | $322.00 | |||
Haptoglobin β Antibody (F-8) FITC | sc-390962 FITC | 200 µg/ml | $336.00 | |||
Haptoglobin β Antibody (F-8) PE | sc-390962 PE | 200 µg/ml | $349.00 | |||
Haptoglobin β Antibody (F-8) Alexa Fluor® 488 | sc-390962 AF488 | 200 µg/ml | $364.00 | |||
Haptoglobin β Antibody (F-8) Alexa Fluor® 546 | sc-390962 AF546 | 200 µg/ml | $364.00 | |||
Haptoglobin β Antibody (F-8) Alexa Fluor® 594 | sc-390962 AF594 | 200 µg/ml | $364.00 | |||
Haptoglobin β Antibody (F-8) Alexa Fluor® 647 | sc-390962 AF647 | 200 µg/ml | $364.00 | |||
Haptoglobin β Antibody (F-8) Alexa Fluor® 680 | sc-390962 AF680 | 200 µg/ml | $364.00 | |||
Haptoglobin β Antibody (F-8) Alexa Fluor® 790 | sc-390962 AF790 | 200 µg/ml | $364.00 |