HAP1 Antibody (C-3): sc-166245

HAP1 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects HAP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HAP1 Antibody (C-3) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HAP1, or huntingtin-associated protein 1, plays a crucial role in neuronal function by binding to huntingtin, a protein containing a polyglutamine region. An expanded polyglutamine repeat in huntingtin exceeding 35 repeats is associated with Huntington′s disease, a neurodegenerative disorder. HAP1′s binding to huntingtin increases in the presence of this expanded region, highlighting HAP1′s potential role in disease pathogenesis. HAP1 exhibits distinct neuronal localization, moving along nerve fibers with huntingtin, and is primarily expressed in brain tissue, particularly in the olfactory bulb and brain stem. In rat models, HAP1 associates with various intracellular organelles, while in mouse neurons, HAP1 localizes to membrane-bound organelles, including large endosomes, tubulovesicular structures, and budding vesicles, emphasizing HAP1′s importance in intracellular transport and signaling pathways.

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HAP1 Antibody (C-3) References:

1. Nuclear and neuropil aggregates in Huntington's disease: relationship to neuropathology.  |  Gutekunst, CA., et al. 1999. J Neurosci. 19: 2522-34. PMID: 10087066
2. A huntingtin-associated protein enriched in brain with implications for pathology.  |  Li, XJ., et al. 1995. Nature. 378: 398-402. PMID: 7477378
3. A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes. The Huntington's Disease Collaborative Research Group.  |  . 1993. Cell. 72: 971-83. PMID: 8458085
4. Huntingtin-associated protein (HAP1): discrete neuronal localizations in the brain resemble those of neuronal nitric oxide synthase.  |  Li, XJ., et al. 1996. Proc Natl Acad Sci U S A. 93: 4839-44. PMID: 8643490
5. Fast transport and retrograde movement of huntingtin and HAP 1 in axons.  |  Block-Galarza, J., et al. 1997. Neuroreport. 8: 2247-51. PMID: 9243620
6. Huntington's disease.  |  Gusella, JF., et al. 1996. Cold Spring Harb Symp Quant Biol. 61: 615-26. PMID: 9246488
7. Analysis of Huntingtin-associated protein 1 in mouse brain and immortalized striatal neurons.  |  Martin, EJ., et al. 1999. J Comp Neurol. 403: 421-30. PMID: 9888310

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HAP1 Antibody (C-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HAP1 Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HAP1 Antibody (C-3) Specifications

Alternate Product Name: HAP1

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 329-628

Epitope Target: HAP1B

Epitope Species: mouse

Product Reactivity/Detection: HAP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HAP1 Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin