hamartin Antibody (A-2): sc-514817

hamartin Antibody (A-2) is a mouse monoclonal IgG2a antibody that detects hamartin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Hamartin plays a crucial role in the regulation of cell growth and proliferation, particularly through interaction with tuberin to form a cytoplasmic complex essential for proper functioning of the mTOR signaling pathway. This pathway is vital for cellular processes such as protein synthesis, autophagy, and metabolism, and dysregulation is implicated in various diseases, including cancer and tuberous sclerosis complex. Hamartin expression in most adult tissues, especially in the brain, heart, and kidney, highlights importance in maintaining tissue homeostasis and function. Furthermore, hamartin′s interaction with the ezrin-radixin-moesin (ERM) family of actin-binding proteins underscores a role in cell adhesion and cytoskeletal organization, making anti-hamartin antibody (A-2) a significant tool for research in developmental biology and disease mechanisms.

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hamartin Antibody (A-2) References:

1. The expression of hamartin, the product of the TSC1 gene, in normal human tissues and in TSC1- and TSC2-linked angiomyolipomas.  |  Plank, TL., et al. 1999. Mod Pathol. 12: 539-45. PMID: 10349994
2. The tuberous sclerosis-1 (TSC1) gene product hamartin suppresses cell growth and augments the expression of the TSC2 product tuberin by inhibiting its ubiquitination.  |  Benvenuto, G., et al. 2000. Oncogene. 19: 6306-16. PMID: 11175345
3. Pathological mutations in TSC1 and TSC2 disrupt the interaction between hamartin and tuberin.  |  Hodges, AK., et al. 2001. Hum Mol Genet. 10: 2899-905. PMID: 11741833
4. Cell cycle-regulated phosphorylation of hamartin, the product of the tuberous sclerosis complex 1 gene, by cyclin-dependent kinase 1/cyclin B.  |  Astrinidis, A., et al. 2003. J Biol Chem. 278: 51372-9. PMID: 14551205
5. Hamartin and tuberin immunohistochemical expression in cutaneous fibroepithelial polyps.  |  Wu, J., et al. 2004. J Cutan Pathol. 31: 383-7. PMID: 15059224
6. Hamartin, the tuberous sclerosis complex 1 gene product, interacts with polo-like kinase 1 in a phosphorylation-dependent manner.  |  Astrinidis, A., et al. 2006. Hum Mol Genet. 15: 287-97. PMID: 16339216
7. Hamartin variants that are frequent in focal dysplasias and cortical tubers have reduced tuberin binding and aberrant subcellular distribution in vitro.  |  Lugnier, C., et al. 2009. J Neuropathol Exp Neurol. 68: 1136-46. PMID: 19918125
8. Phosphorylated hamartin-Hsp70 complex regulates apoptosis via mitochondrial localization.  |  Inoue, H., et al. 2010. Biochem Biophys Res Commun. 391: 1148-53. PMID: 20006582
9. Minute amounts of hamartin wildtype rescue the emergence of tuber-like lesions in conditional Tsc1 ablated mice.  |  Robens, BK., et al. 2016. Neurobiol Dis. 95: 134-44. PMID: 27425891
10. Hamartin, the product of the tuberous sclerosis 1 (TSC1) gene, interacts with tuberin and appears to be localized to cytoplasmic vesicles.  |  Plank, TL., et al. 1998. Cancer Res. 58: 4766-70. PMID: 9809973

The preferred hamartin antibody is hamartin Antibody (C-8): sc-377386.