Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HADHB CRISPR/Cas9 KO Plasmid (m): sc-433034
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HADHB CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HADHB genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HADHB Antibody (E-1): sc-271495
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HADHB CRISPR/Cas9 KO Plasmid (m) | sc-433034 | 20 µg | $397.00 |
Overview
Mouse Hadhb encodes the beta subunit of the mitochondrial trifunctional protein (HADHB), an essential enzyme complex that catalyzes key steps of long-chain fatty acid β-oxidation, including enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase activities. By supporting mitochondrial energy metabolism, HADHB helps maintain lipid homeostasis and influences cellular responses to nutrient availability and metabolic stress. Disruption of mitochondrial fatty acid oxidation can perturb redox balance and ATP production, with downstream effects on tissues with high oxidative demand. Altered HADHB function is therefore relevant to studies of mitochondrial dysfunction and lipid-associated pathology, including cardiometabolic and neuromuscular phenotypes modeled in mice.
HADHB CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hadhb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hadhb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hadhb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HADHB protein expression.
This CRISPR knockout system enables efficient generation of Hadhb-deficient cell models for investigation of HADHB signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hadhb exon(s) critical for HADHB function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hadhb genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HADHB CRISPR/Cas9 KO Plasmid (m) and HADHB CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hadhb locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HADHB HDR Plasmid (m) and HADHB HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hadhb homology arms to support homology-directed repair at defined Hadhb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.