HADHB Antibody (E-1): sc-271495

HADHB Antibody (E-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects HADHB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HADHB (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HADHB protein, also known as Trifunctional enzyme subunit beta (mitochondrial) and Acetyl-CoA acyltransferase, is a crucial 474 amino acid protein encoded by the human gene HADHB. HADHB protein plays a vital role in mitochondrial fatty acid oxidation, functioning as part of a larger trifunctional protein complex that catalyzes the breakdown of long-chain fatty acids into acetyl-CoA, which is essential for energy production. HADHB protein is located in the mitochondrion, forming an octameric structure composed of four alpha (HADHA) and four beta (HADHB) subunits, which is critical for enzymatic activity and stability. Deficiencies in HADHB can lead to trifunctional protein deficiency, a condition characterized by hypoglycemia, cardiomyopathy, and sudden death, highlighting the importance of HADHB protein in metabolic health. Biochemically, trifunctional protein deficiency is defined by the loss of all three enzyme activities of the trifunctional protein complex, underscoring the significance of HADHB in maintaining proper metabolic function.

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HADHB Antibody (E-1) References:

1. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.  |  Gevaert, K., et al. 2003. Nat Biotechnol. 21: 566-9. PMID: 12665801
2. Molecular and phenotypic heterogeneity in mitochondrial trifunctional protein deficiency due to beta-subunit mutations.  |  Spiekerkoetter, U., et al. 2003. Hum Mutat. 21: 598-607. PMID: 12754706
3. HADHB, HuR, and CP1 bind to the distal 3'-untranslated region of human renin mRNA and differentially modulate renin expression.  |  Adams, DJ., et al. 2003. J Biol Chem. 278: 44894-903. PMID: 12933794
4. cAMP controls human renin mRNA stability via specific RNA-binding proteins.  |  Morris, BJ., et al. 2004. Acta Physiol Scand. 181: 369-73. PMID: 15283747
5. Isolated mitochondrial long-chain ketoacyl-CoA thiolase deficiency resulting from mutations in the HADHB gene.  |  Das, AM., et al. 2006. Clin Chem. 52: 530-4. PMID: 16423905
6. HADHB mutations cause infantile-onset axonal Charcot-Marie-Tooth disease: A report of two cases.  |  Lu, Y., et al. 2018. Clin Neuropathol. 37: 232-238. PMID: 29956646
7. Novel HADHB mutations in a patient with mitochondrial trifunctional protein deficiency.  |  Nakama, M., et al. 2020. Hum Genome Var. 7: 10. PMID: 32257295
8. HADHB, a fatty acid beta-oxidation enzyme, is a potential prognostic predictor in malignant lymphoma.  |  Sekine, Y., et al. 2022. Pathology. 54: 286-293. PMID: 34531036
9. Identification of ACAA1 and HADHB as potential prognostic biomarkers based on a novel fatty acid oxidation-related gene model in head and neck squamous cell carcinoma: A retrospective study.  |  Wang, WL., et al. 2024. Arch Oral Biol. 163: 105982. PMID: 38678878
10. DNA demethylase TET2-mediated reduction of HADHB expression contributes to cadmium-induced malignant progression of colorectal cancer.  |  Li, L., et al. 2024. Ecotoxicol Environ Saf. 280: 116579. PMID: 38865940
11. Molecular characterization of mitochondrial trifunctional protein deficiency: formation of the enzyme complex is important for stabilization of both alpha- and beta-subunits.  |  Ushikubo, S., et al. 1996. Am J Hum Genet. 58: 979-88. PMID: 8651282
12. Fluorescence in situ hybridization mapping of the alpha and beta subunits (HADHA and HADHB) of human mitochondrial fatty acid beta-oxidation multienzyme complex to 2p23 and their evolution.  |  Aoyama, T., et al. 1997. Cytogenet Cell Genet. 79: 221-4. PMID: 9605857

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HADHB Antibody (E-1) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-HADHB Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HADHB Antibody (E-1) Specifications

Alternate Product Name: HADHB

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-290

Epitope Target: HADHB

Epitope Species: human

Product Reactivity/Detection: HADHB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for HADHB Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin