HADHA Antibody (E-8): sc-374497

HADHA Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects HADHA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HADHA Antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HADHA, also known as Trifunctional enzyme subunit alpha, mitochondrial, is a crucial component of the mitochondrial trifunctional protein complex, which plays a vital role in the final three steps of mitochondrial beta-oxidation of long-chain fatty acids. This complex is composed of four alpha (HADHA) and four beta (HADHB) subunits, with HADHA specifically responsible for the enzymatic activities of 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase. Proper functioning of HADHA is essential for efficient fatty acid metabolism, and mutations in HADHA can lead to severe metabolic disorders such as long-chain 3-hydroxyacyl-coenzyme A dehydrogenase deficiency and mitochondrial trifunctional protein deficiency, both of which can result in significant health complications. Anti-HADHA antibody (E-8) allows researchers to explore the implications of these deficiencies and the overall importance of fatty acid oxidation in cellular energy production and metabolic health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HADHA Antibody (E-8) References:

1. Uniparental disomy of chromosome 2 resulting in lethal trifunctional protein deficiency due to homozygous alpha-subunit mutations.  |  Spiekerkoetter, U., et al. 2002. Hum Mutat. 20: 447-51. PMID: 12442268
2. Isolated mitochondrial long-chain ketoacyl-CoA thiolase deficiency resulting from mutations in the HADHB gene.  |  Das, AM., et al. 2006. Clin Chem. 52: 530-4. PMID: 16423905
3. Identification of a novel single nucleotide polymorphism of HADHA gene at a referred primer-binding site during pre-diagnostic tests for preimplantation genetic diagnosis.  |  Lee, HS., et al. 2006. J Korean Med Sci. 21: 794-9. PMID: 17043408
4. ENU mutagenesis identifies mice with cardiac fibrosis and hepatic steatosis caused by a mutation in the mitochondrial trifunctional protein beta-subunit.  |  Kao, HJ., et al. 2006. Hum Mol Genet. 15: 3569-77. PMID: 17116638
5. Identification of novel mutations of the HADHA and HADHB genes in patients with mitochondrial trifunctional protein deficiency.  |  Choi, JH., et al. 2007. Int J Mol Med. 19: 81-7. PMID: 17143551
6. Acute fatty liver of pregnancy and neonatal long-chain 3-hydroxyacyl-coenzyme A dehydrogenase (LCHAD) deficiency.  |  Gutiérrez Junquera, C., et al. 2009. Eur J Pediatr. 168: 103-6. PMID: 18408953
7. [Long-chain 3-hydroxyacyl CoA dehydrogenase deficiency and choroidal neovascularization].  |  Stopek, D., et al. 2008. J Fr Ophtalmol. 31: 993-8. PMID: 19107076
8. MiR-612 regulates invadopodia of hepatocellular carcinoma by HADHA-mediated lipid reprogramming.  |  Liu, Y., et al. 2020. J Hematol Oncol. 13: 12. PMID: 32033570
9. The mitochondrial β-oxidation enzyme HADHA restrains hepatic glucagon response by promoting β-hydroxybutyrate production.  |  Pan, A., et al. 2022. Nat Commun. 13: 386. PMID: 35046401
10. Zfp335 establishes eTreg lineage and neonatal immune tolerance by targeting Hadha-mediated fatty acid oxidation.  |  Wang, X., et al. 2023. J Clin Invest. 133: PMID: 37843279
11. KDM6B-Mediated HADHA Demethylation/Lactylation Regulates Cementogenesis.  |  Yang, Z., et al. 2025. J Dent Res. 104: 75-85. PMID: 39569625
12. Fluorescence in situ hybridization mapping of the alpha and beta subunits (HADHA and HADHB) of human mitochondrial fatty acid beta-oxidation multienzyme complex to 2p23 and their evolution.  |  Aoyama, T., et al. 1997. Cytogenet Cell Genet. 79: 221-4. PMID: 9605857

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HADHA Antibody (E-8) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-HADHA Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HADHA Antibody (E-8) Specifications

Alternate Product Name: HADHA

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 481-763

Epitope Target: HADHA

Epitope Species: human

Product Reactivity/Detection: HADHA

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for HADHA Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin