H2-K^k Antibody (AF3-12.1): sc-53851

H2-Kk Antibody (AF3-12.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the H2-Kk protein of mouse origin by flow cytometry (FCM). Anti-H2-Kk antibody (AF3-12.1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. H2-Kk is a crucial component of the major histocompatibility complex (MHC) class I molecules, which play a vital role in the immune response by presenting endogenous peptides to cytotoxic T lymphocytes (CTLs). This presentation is essential for the recognition and elimination of infected or malignant cells, thereby maintaining cellular integrity and immune surveillance. H2-Kk is predominantly expressed on the surface of L929 fibroblasts, and interaction with T cell receptors is critical for the activation of CTLs, which are pivotal in orchestrating adaptive immunity. The structural characteristics of H2-Kk, including association with β-2-Microglobulin, enable effective binding and presentation of a diverse array of peptide antigens, making H2-Kk an important target for studies related to immune response and potential therapeutic interventions.

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H2-K^k Antibody (AF3-12.1) References:

1. The nature of the MHC class I peptide loading complex.  |  Cresswell, P., et al. 1999. Immunol Rev. 172: 21-8. PMID: 10631934
2. Jun oncoproteins do not function as primary transcription factors for the mouse major histocompatibility complex class I H-2 genes in fibroblasts.  |  Hatina, J. and Reischig, J. 2003. Eur J Immunogenet. 30: 253-7. PMID: 12919286
3. A molecular model of MHC class-I-restricted antigen processing.  |  Monaco, JJ. 1992. Immunol Today. 13: 173-9. PMID: 1386516
4. Major histocompatibility complex antigens in v-Ki-ras transformed cells: the different antigens are expressed and induced by interferons independently of one another and of the anti-viral state.  |  Morris, AG. 1990. Immunology. 71: 224-9. PMID: 1699881
5. Reduced tumorigenicity of murine tumor cells secreting gamma-interferon is due to nonspecific host responses and is unrelated to class I major histocompatibility complex expression.  |  Esumi, N., et al. 1991. Cancer Res. 51: 1185-9. PMID: 1900037
6. Identification of glycoproteins carrying a target glycan-motif by liquid chromatography/multiple-stage mass spectrometry: identification of Lewis x-conjugated glycoproteins in mouse kidney.  |  Hashii, N., et al. 2009. J Proteome Res. 8: 3415-29. PMID: 19453144
7. Antigen-specific recognition is critical for the function of regulatory CD8(+)CD28(-) T cells.  |  Renner, P., et al. 2010. Transpl Immunol. 22: 144-9. PMID: 19840851
8. Purification of the H-2Kk molecule of the murine major histocompatibility complex.  |  Herrmann, SH. and Mescher, MF. 1979. J Biol Chem. 254: 8713-6. PMID: 479148
9. H2Kk can influence whether cytotoxic T lymphocytes recognize trinitrophenyl in association with H2Dk unique or H-2Kk and H-2Dk shared self determinants.  |  Fujiwara, H., et al. 1982. J Immunol. 129: 1189-93. PMID: 6179996
10. Peptides naturally presented by MHC class I molecules.  |  Rammensee, HG., et al. 1993. Annu Rev Immunol. 11: 213-44. PMID: 8476560
11. An acidic modification of the cytoplasmic domain contributes to the charge heterogeneity of the MHC class I antigens.  |  Balomenos, D. and Poretz, RD. 1998. Immunogenetics. 47: 381-9. PMID: 9510556

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-H2-K^k Antibody (AF3-12.1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-H2-K^k Antibody (AF3-12.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

H2-K^k Antibody (AF3-12.1) Specifications

Alternate Product Name: H2-Kk

Product Clone Name: AF3-12.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: H2-K^k

Epitope Species: mouse

Product Reactivity/Detection: MHC class I H2-K^k

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for H2-K^k Antibody (AF3-12.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin