H2-Kk Antibody (AF3-12.1) recommended for detection of MHC class I H2-Kk of mouse origin by FCM

H2-Kk Antibody (AF3-12.1): sc-53851

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H2-Kk Antibody (AF3-12.1) is rated 2.0 out of 5 by 1.
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Datasheets

    • Anti-H2-Kk Antibody (AF3-12.1) is a mouse monoclonal IgG1 (kappa light chain) H2-Kk antibody provided at 200 µg/ml
    • raised against A/J spelenocytes of mouse origin
    • H2-Kk Antibody (AF3-12.1) is recommended for detection of MHC class I H2-Kk of mouse origin by FCM
    • Anti-H2-Kk Antibody (AF3-12.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of H2-Kk (AF3-12.1): sc-53851.

How can H2-Kk (AF3-12.1): sc-53851 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Randy McDonald
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53851 PE) or fluoroscein (sc-53851 FITC).
Answered by: Technical Support
Date published: 2017-02-28
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