H⁺/K⁺ ATPase β Antibody (H-7): sc-376385

H+/K+ ATPase β Antibody (H-7) is a mouse monoclonal IgG1 antibody that detects H+/K+ ATPase β in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). H+/K+ ATPase β, also known as ATP4B or ATP6B, is a crucial 291 amino acid single-pass type II membrane protein that serves as the β subunit of the H+/K+ ATPase heterodimer, working in concert with the α subunit to facilitate ATP hydrolysis while exchanging hydrogen and potassium ions across the plasma membrane. This process is vital for gastric acid secretion, which is essential for digestion and maintaining the acidic environment of the stomach. Proper functioning of H+/K+ ATPase β is critical, as dysregulation can lead to various gastrointestinal disorders, including acid reflux and peptic ulcers. Additionally, the gene encoding H+/K+ ATPase β is located on human chromosome 13, a region that is significant not only for its role in encoding over 400 genes, including tumor suppressor genes like BRCA2 and RB1, but also for its association with serious genetic conditions such as Patau syndrome, which can result in severe developmental issues and increased susceptibility to infections. Anti-H+/K+ ATPase β antibody (H-7) is an invaluable tool for researchers studying gastric physiology and related pathologies.

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H⁺/K⁺ ATPase β Antibody (H-7) References:

1. Monoclonal antibodies specific for the core protein of the beta-subunit of the gastric proton pump (H+/K+ ATPase). An autoantigen targetted in pernicious anaemia.  |  Jones, CM., et al. 1991. Eur J Biochem. 197: 49-59. PMID: 1707813
2. The 60- to 90-kDa parietal cell autoantigen associated with autoimmune gastritis is a beta subunit of the gastric H+/K(+)-ATPase (proton pump).  |  Toh, BH., et al. 1990. Proc Natl Acad Sci U S A. 87: 6418-22. PMID: 1974721
3. Atp4b promoter directs the expression of Cre recombinase in gastric parietal cells of transgenic mice.  |  Zhao, Z., et al. 2010. J Genet Genomics. 37: 647-52. PMID: 20933217
4. Characterization of a beta subunit of the gastric H+/K(+)-transporting ATPase.  |  Reuben, MA., et al. 1990. Proc Natl Acad Sci U S A. 87: 6767-71. PMID: 2168558
5. The beta-subunit of the gastric H+/K(+)-ATPase can occur without the alpha-subunit.  |  Baldwin, GS. 1990. FEBS Lett. 272: 159-62. PMID: 2172012
6. Intragenic DNA methylation concomitant with repression of ATP4B and ATP4A gene expression in gastric cancer is a potential serum biomarker.  |  Raja, UM., et al. 2012. Asian Pac J Cancer Prev. 13: 5563-8. PMID: 23317218
7. ESRRG, ATP4A, and ATP4B as Diagnostic Biomarkers for Gastric Cancer: A Bioinformatic Analysis Based on Machine Learning.  |  Chen, Q., et al. 2022. Front Physiol. 13: 905523. PMID: 35812327
8. Functional significance of the beta-subunit for heterodimeric P-type ATPases.  |  Chow, DC. and Forte, JG. 1995. J Exp Biol. 198: 1-17. PMID: 7891030
9. Renal expression of the gene encoding the gastric H(+)-K(+)-ATPase beta-subunit.  |  Callaghan, JM., et al. 1995. Am J Physiol. 268: F363-74. PMID: 7900835
10. Expression of the gastric H/K-ATPase alpha-subunit in the thymus may explain the dominant role of the beta-subunit in the pathogenesis of autoimmune gastritis.  |  Alderuccio, F., et al. 1997. Autoimmunity. 25: 167-75. PMID: 9272282

The preferred H⁺/K⁺ ATPase β antibody is H⁺/K⁺ ATPase β Antibody (C-4): sc-374094.