H-Caldesmon Antibody (h-CALD): sc-58703

H-Caldesmon Antibody (h-CALD) is a mouse monoclonal IgG1 kappa light chain antibody that detects the H-Caldesmon protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-H-Caldesmon antibody (h-CALD) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. H-Caldesmon, also known as high molecular weight Caldesmon, Caldesmon isoform 1, and muscular Caldesmon, is primarily located in the thin filaments of smooth muscle cells, where H-Caldesmon plays a crucial role in regulating cellular contraction, exocytosis, endocytosis, cell movement, and changes in cell shape. This localization is significant because H-Caldesmon interacts with actin and calmodulin, thereby influencing the contractile properties of smooth muscle and contributing to various physiological processes. Notably, while H-Caldesmon is expressed in smooth muscle tumors of soft tissue, H-Caldesmon is absent in myofibroblasts, making H-Caldesmon a valuable marker for differentiating smooth muscle tumors from other neoplasms, such as bone tumors with myoid differentiation and epithelioid mesothelioma versus lung adenocarcinoma.

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H-Caldesmon Antibody (h-CALD) References:

1. h-Caldesmon as a specific marker for smooth muscle tumors. Comparison with other smooth muscle markers in bone tumors.  |  Watanabe, K., et al. 2000. Am J Clin Pathol. 113: 663-8. PMID: 10800398
2. Calponin and h-caldesmon expression in atypical fibroxanthoma and superficial leiomyosarcoma.  |  Sakamoto, A., et al. 2002. Virchows Arch. 440: 404-9. PMID: 11956822
3. h-Caldesmon as a specific marker of smooth muscle cell differentiation in some soft tissue tumors of the skin.  |  D'Addario, SF., et al. 2002. J Cutan Pathol. 29: 426-9. PMID: 12139638
4. H-caldesmon expression in myofibroblastoma of the breast: evidence supporting the distinction from leiomyoma.  |  Magro, G., et al. 2003. Histopathology. 42: 233-8. PMID: 12605642
5. Reduced expression of actin-binding proteins, h-caldesmon and calponin h1, in the vascular smooth muscle inside melanoma lesions: an adverse prognostic factor for malignant melanoma.  |  Koganehira, Y., et al. 2003. Br J Dermatol. 148: 971-80. PMID: 12786828
6. Phenotypic changes of human smooth muscle cells during development: late expression of heavy caldesmon and calponin.  |  Frid, MG., et al. 1992. Dev Biol. 153: 185-93. PMID: 1397676
7. Comparative study of CD34, alpha-SMA and h-caldesmon expression in the stroma of gynaecomastia and male breast carcinoma.  |  Kalekou, H., et al. 2005. Histopathology. 47: 74-81. PMID: 15982326
8. Differential expression of smooth muscle myosin, smooth muscle actin, h-caldesmon, and calponin in the diagnosis of myofibroblastic and smooth muscle lesions of skin and soft tissue.  |  Perez-Montiel, MD., et al. 2006. Am J Dermatopathol. 28: 105-11. PMID: 16625070
9. h-Caldesmon, a useful positive marker in the diagnosis of pleural malignant mesothelioma, epithelioid type.  |  Comin, CE., et al. 2006. Am J Surg Pathol. 30: 463-9. PMID: 16625092
10. Transforming growth factor-beta1 antisense treatment of rat vein grafts reduces the accumulation of collagen and increases the accumulation of h-caldesmon.  |  Wolff, RA., et al. 2006. J Vasc Surg. 43: 1028-36. PMID: 16678700
11. h-caldesmon immunoreactivity in atypical fibroxanthoma: implications for the differential diagnosis.  |  Martinez-Ciarpaglini, C., et al. 2018. Pathology. 50: 358-361. PMID: 29490873
12. Co-expression of CD34 and h-caldesmon in a benign meningioma-like dermal neoplasm, a case report.  |  Kussaibi, H. 2020. Dermatol Reports. 12: 8994. PMID: 33408843

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-H-Caldesmon Antibody (h-CALD) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-H-Caldesmon Antibody (h-CALD) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

H-Caldesmon Antibody (h-CALD) Specifications

Alternate Product Name: H-Caldesmon

Product Clone Name: h-CALD

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: H-Caldesmon

Epitope Species: human

Product Reactivity/Detection: H-Caldesmon

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for H-Caldesmon Antibody (h-CALD): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin