GW182 CRISPR/Cas9 KO Plasmid (h): sc-400906
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GW182 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GW182 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GW182 Antibody (A-6): sc-374458
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GW182 CRISPR/Cas9 KO Plasmid (h) | sc-400906 | 20 µg | $397.00 |
Overview
TNRC6A encodes GW182, a core scaffolding component of the microRNA-induced silencing complex that coordinates post-transcriptional gene repression. GW182 binds Argonaute proteins and recruits CCR4–NOT and PAN2–PAN3 deadenylase complexes, promoting mRNA deadenylation, decapping, and translational inhibition within cytoplasmic P-bodies. Through these interactions, TNRC6A helps shape microRNA-controlled gene expression programs that influence cell-cycle control, differentiation, stress responses, and innate immune signaling. Dysregulation of GW182-associated silencing networks has been linked to aberrant transcriptome regulation observed across multiple disease contexts, including cancer and neurological disorders, making TNRC6A a useful node for mechanistic RNA biology studies.
GW182 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TNRC6A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TNRC6A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TNRC6A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GW182 protein expression.
This CRISPR knockout system enables efficient generation of TNRC6A-deficient cell models for investigation of GW182 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TNRC6A exon(s) critical for GW182 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TNRC6A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GW182 CRISPR/Cas9 KO Plasmid (h) and GW182 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TNRC6A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GW182 HDR Plasmid (h) and GW182 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TNRC6A homology arms to support homology-directed repair at defined TNRC6A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.