GW182 Antibody (A-6): sc-374458
- GW182 Antibody (A-6) is a mouse monoclonal IgM κ GW182 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1555-1593 within an internal region of GW182 of human origin
- recommended for detection of GW182 isoforms 1, 2 and 3 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and avian
- available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GW182 Antibody (A-6). This work is in progress.
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GW182 Antibody (A-6) is a mouse monoclonal IgM antibody that detects GW182 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-GW182 antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GW182 plays a critical role in gene expression regulation by interacting with microRNAs (miRNAs) and is essential for the formation and function of processing bodies (P-bodies), which are involved in mRNA decay and storage. P-bodies are vital for maintaining cellular homeostasis and ensuring proper mRNA regulation, as disassembly can lead to impaired silencing of target mRNAs, potentially resulting in various diseases. GW182 serves as a cytoplasmic marker for these structures and interacts with argonaute-2, a key component of the RNA-induced silencing complex. GW182 has been associated with autoimmune conditions such as ataxia and Sjögren′s syndrome, where patients develop autoantibodies against proteins associated with P-bodies, highlighting GW182′s importance in both normal cellular function and disease pathology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GW182 Antibody (A-6) References:
- mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes. | Behm-Ansmant, I., et al. 2006. Genes Dev. 20: 1885-98. PMID: 16815998
- GW182 family proteins are crucial for microRNA-mediated gene silencing. | Ding, L. and Han, M. 2007. Trends Cell Biol. 17: 411-6. PMID: 17766119
- Identification of GW182 and its novel isoform TNGW1 as translational repressors in Ago2-mediated silencing. | Li, S., et al. 2008. J Cell Sci. 121: 4134-44. PMID: 19056672
- Optimization of immunoprecipitation-western blot analysis in detecting GW182-associated components of GW/P bodies. | Moser, JJ., et al. 2009. Nat Protoc. 4: 674-85. PMID: 19373232
- A C-terminal silencing domain in GW182 is essential for miRNA function. | Eulalio, A., et al. 2009. RNA. 15: 1067-77. PMID: 19383769
- Mammalian GW182 contains multiple Argonaute-binding sites and functions in microRNA-mediated translational repression. | Takimoto, K., et al. 2009. RNA. 15: 1078-89. PMID: 19398495
- The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets. | Huntzinger, E., et al. 2013. Nucleic Acids Res. 41: 978-94. PMID: 23172285
- Structural features of Argonaute-GW182 protein interactions. | Pfaff, J., et al. 2013. Proc Natl Acad Sci U S A. 110: E3770-9. PMID: 24043833
- Dcp1a and GW182 Induce Distinct Cellular Aggregates and Have Different Effects on microRNA Pathway. | Wang, X., et al. 2017. DNA Cell Biol. 36: 565-570. PMID: 28488892
- GW182 Proteins Restrict Extracellular Vesicle-Mediated Export of MicroRNAs in Mammalian Cancer Cells. | Ghosh, S., et al. 2021. Mol Cell Biol. 41: PMID: 33685914
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GW182 Antibody (A-6) | sc-374458 | 200 µg/ml | $316.00 | |||
GW182 Antibody (A-6) AC | sc-374458 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GW182 Antibody (A-6) Alexa Fluor® 488 | sc-374458 AF488 | 200 µg/ml | $357.00 | |||
GW182 Antibody (A-6) Alexa Fluor® 647 | sc-374458 AF647 | 200 µg/ml | $357.00 | |||
GW182 Antibody (A-6) FITC | sc-374458 FITC | 200 µg/ml | $330.00 | |||
GW182 Antibody (A-6) HRP | sc-374458 HRP | 200 µg/ml | $316.00 | |||
GW182 (A-6) Neutralizing Peptide | sc-374458 P | 100 µg/0.5 ml | $68.00 | |||
GW182 Antibody (A-6) PE | sc-374458 PE | 200 µg/ml | $343.00 |