GW182 Antibody (2D6): sc-56313
- GW182 Antibody (2D6) is a mouse monoclonal IgM κ, cited in 3 publications, provided at 200 µg/ml
- raised against partial length GW182 of human origin
- recommended for detection of GW182 of human origin by IF and IHC(P)
- See GW182 (A-6): sc-374458 for GW182 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GW182 Antibody (2D6). This work is in progress.
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GW182 Antibody (2D6) is a mouse monoclonal IgM antibody that detects GW182 in human samples through immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP). GW182 plays a pivotal role in the formation and maintenance of GW bodies (GWBs), which are essential for the regulation of microRNA (miRNA) and small interfering RNA (siRNA) activities within the cell. These structures serve as critical storage and degradation sites for mRNAs, and their integrity is vital for effective gene silencing. Disruption of GWBs can lead to impaired silencing activity, which has significant implications in various diseases, including ataxia and Sjögren′s syndrome, where patients develop autoantibodies against GWB proteins. GW182, also known as the EMSY interactor protein, is ubiquitously expressed and interacts with endogenous argonaute-2 (Ago2), further emphasizing GW182′s importance in RNA regulation. Anti-GW182 antibody (2D6) serves as an invaluable tool for researchers studying the dynamics of RNA processing and the pathological mechanisms underlying diseases associated with GWB dysfunction.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GW182 Antibody (2D6) References:
- A panel of monoclonal antibodies to cytoplasmic GW bodies and the mRNA binding protein GW182. | Eystathioy, T., et al. 2003. Hybrid Hybridomics. 22: 79-86. PMID: 12831532
- The GW182 protein family in animal cells: new insights into domains required for miRNA-mediated gene silencing. | Eulalio, A., et al. 2009. RNA. 15: 1433-42. PMID: 19535464
- Function of GW182 and GW bodies in siRNA and miRNA pathways. | Yao, B., et al. 2013. Adv Exp Med Biol. 768: 71-96. PMID: 23224966
- The role of GW182 proteins in miRNA-mediated gene silencing. | Braun, JE., et al. 2013. Adv Exp Med Biol. 768: 147-63. PMID: 23224969
- Role of GW182 protein in the cell. | Niaz, S. and Hussain, MU. 2018. Int J Biochem Cell Biol. 101: 29-38. PMID: 29791863
- The Requirement for GW182 Scaffolding Protein Depends on Whether Argonaute Is Mediating Translation, Transcription, or Splicing. | Liu, J., et al. 2018. Biochemistry. 57: 5247-5256. PMID: 30086238
- Distinct temporal expression of the GW182 paralog TNRC6A in neurons regulates dendritic arborization. | Nawalpuri, B., et al. 2021. J Cell Sci. 134: PMID: 34328181
- N-terminal Ago-binding domain of GW182 contains a tryptophan-rich region that confer binding to the CCR4-NOT complex. | Wakiyama, M. and Takimoto, K. 2022. Genes Cells. 27: 579-585. PMID: 35822830
- Convergence of multiple RNA-silencing pathways on GW182/TNRC6. | Welte, T., et al. 2023. Mol Cell. 83: 2478-2492.e8. PMID: 37369201
- RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules. | Majerciak, V., et al. 2023. Nucleic Acids Res. 51: 9337-9355. PMID: 37427791
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GW182 Antibody (2D6) | sc-56313 | 200 µg/ml | $322.00 |