Gγ 5 CRISPR/Cas9 KO Plasmid (h): sc-407524
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gγ 5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Gγ 5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gγ 5 Antibody (3B8): sc-517161
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gγ 5 CRISPR/Cas9 KO Plasmid (h) | sc-407524 | 20 µg | $397.00 |
Overview
GNG5 encodes the human Gγ5 subunit, a core component of heterotrimeric G proteins that couple activated G protein-coupled receptors (GPCRs) to downstream signaling. By partnering with Gβ subunits, Gβγ dimers regulate effector enzymes and ion channels, shaping second-messenger pathways such as adenylyl cyclase/cAMP, phospholipase Cβ/IP3–DAG, and PI3K–AKT signaling. Gγ5-dependent signaling influences processes including cell migration, membrane excitability, and cytoskeletal remodeling, and altered GPCR/G protein pathway activity is frequently implicated in cancer biology, inflammation, and cardiovascular and neurological dysfunction.
Gγ 5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GNG5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GNG5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GNG5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Gγ 5 protein expression.
This CRISPR knockout system enables efficient generation of GNG5-deficient cell models for investigation of Gγ 5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GNG5 exon(s) critical for Gγ 5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GNG5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Gγ 5 CRISPR/Cas9 KO Plasmid (h) and Gγ 5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GNG5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Gγ 5 HDR Plasmid (h) and Gγ 5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GNG5 homology arms to support homology-directed repair at defined GNG5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.