Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Gα 15 CRISPR/Cas9 KO Plasmid (h2): sc-403266-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gα 15 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Gα 15 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gα 15 Antibody (F-3): sc-393878
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gα 15 CRISPR/Cas9 KO Plasmid (h2) | sc-403266-KO-2 | 20 µg | $397.00 |
Overview
GNA15 encodes the human heterotrimeric G protein alpha subunit Gα15, a promiscuous Gq-family mediator that couples diverse G protein–coupled receptors to phospholipase Cβ activation. Upon receptor engagement, Gα15 promotes inositol trisphosphate and diacylglycerol signaling, intracellular Ca²⁺ mobilization, protein kinase C activity, and downstream transcriptional programs that shape secretion, chemotaxis, and inflammatory responses. This signaling axis intersects with MAPK and other second-messenger pathways, influencing cell activation states across immune and hematopoietic contexts. Altered GPCR–Gα15 coupling and dysregulated calcium-dependent signaling have been implicated in cancer- and inflammation-associated phenotypes, supporting its use in mechanistic studies of receptor rewiring and signal integration.
Gα 15 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the GNA15 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GNA15 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GNA15 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Gα 15 protein expression.
This CRISPR knockout system enables efficient generation of GNA15-deficient cell models for investigation of Gα 15 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GNA15 exon(s) critical for Gα 15 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GNA15 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Gα 15 CRISPR/Cas9 KO Plasmid (h) and Gα 15 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GNA15 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Gα 15 HDR Plasmid (h) and Gα 15 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GNA15 homology arms to support homology-directed repair at defined GNA15 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.