G_{α 15} Antibody (F-3): sc-393878

Gα 15 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects the G alpha 15 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). G alpha 15 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. G alpha 15 plays a crucial role in signal transduction as a member of the Gq subfamily of heterotrimeric G proteins, which are essential for relaying signals from cell surface receptors to intracellular effectors. G alpha 15 is predominantly expressed in hematopoietic cells, where G alpha 15 participates in mediating responses to various extracellular stimuli, such as hormones and neurotransmitters. G alpha 15′s ability to bind and hydrolyze guanosine triphosphate (GTP) is vital for function, as G alpha 15 regulates the activity of downstream effectors, including phospholipase C, which generates inositol trisphosphate and diacylglycerol, leading to increased intracellular calcium levels and activation of protein kinase C. Understanding G alpha 15 function is important for elucidating G alpha 15′s role in hematopoietic cell signaling and potential implications in various physiological and pathological processes, including immune responses and hematopoiesis.

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G_{α 15} Antibody (F-3) References:

1. Cloning and characterization of a rabbit ortholog of human Galpha16 and mouse G(alpha)15.  |  Feild, JA., et al. 1999. FEBS Lett. 460: 53-6. PMID: 10571060
2. Regulation of hematopoietic-specific G-protein Galpha15 and Galpha16 by protein kinase C.  |  Gu, JL., et al. 2003. J Cell Biochem. 88: 1101-11. PMID: 12647293
3. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
4. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
5. Gustducin is a taste-cell-specific G protein closely related to the transducins.  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
6. Development of a universal high-throughput calcium assay for G-protein- coupled receptors with promiscuous G-protein Galpha15/16.  |  Zhu, T., et al. 2008. Acta Pharmacol Sin. 29: 507-16. PMID: 18358098
7. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
8. G alpha 16, a G protein alpha subunit specifically expressed in hematopoietic cells.  |  Amatruda, TT., et al. 1991. Proc Natl Acad Sci U S A. 88: 5587-91. PMID: 1905813
9. Biochemical characterization of three stimulatory GTP-binding proteins. The large and small forms of Gs and the olfactory-specific G-protein, Golf.  |  Jones, DT., et al. 1990. J Biol Chem. 265: 2671-6. PMID: 2105931
10. Gα15 in early onset of pancreatic ductal adenocarcinoma.  |  Innamorati, G., et al. 2021. Sci Rep. 11: 14922. PMID: 34290274
11. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779
12. Identification of two alpha-subunit species of GTP-binding proteins, Galpha15 and Galphaq, expressed in rat taste buds.  |  Kusakabe, Y., et al. 1998. Biochim Biophys Acta. 1403: 265-72. PMID: 9685675

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α 15} Antibody (F-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α 15} Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α 15} Antibody (F-3) Specifications

Alternate Product Name: G protein subunit GNA15

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-45

Epitope Target: G_{α 15}

Epitope Species: human

Product Reactivity/Detection: G_{α 15}

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for G_{α 15} Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred G_{α q/11/14} antibody is G_{α q/11/14} Antibody (G-7): sc-365906.