Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GSTM1 CRISPR/Cas9 KO Plasmid (h): sc-418201
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSTM1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GSTM1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GSTM1 Antibody (1H4F2): sc-517262
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSTM1 CRISPR/Cas9 KO Plasmid (h) | sc-418201 | 20 µg | $397.00 |
Overview
GSTM1 encodes glutathione S-transferase mu 1 (GSTM1), a phase II detoxification enzyme that catalyzes glutathione conjugation to electrophilic xenobiotics and endogenous reactive intermediates. By limiting oxidative stress and lipid peroxidation–derived adducts, GSTM1 contributes to cellular redox homeostasis and supports metabolism of drugs, environmental chemicals, and byproducts of inflammation. Its activity intersects with glutathione-dependent antioxidant pathways and broader cytoprotective stress responses, influencing susceptibility to oxidative DNA damage. Genetic loss or reduced expression of GSTM1 has been associated with inter-individual variability in toxicant handling and has been studied in contexts where oxidative stress and carcinogen exposure contribute to disease risk.
GSTM1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GSTM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GSTM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GSTM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GSTM1 protein expression.
This CRISPR knockout system enables efficient generation of GSTM1-deficient cell models for investigation of GSTM1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GSTM1 exon(s) critical for GSTM1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GSTM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GSTM1 CRISPR/Cas9 KO Plasmid (h) and GSTM1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GSTM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GSTM1 HDR Plasmid (h) and GSTM1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GSTM1 homology arms to support homology-directed repair at defined GSTM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.