GSTA1 Antibody (R-14): sc-100546

GSTA1 Antibody (R-14) is a mouse monoclonal IgG1 kappa light chain antibody that detects GSTA1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-GSTA1 antibody (R-14) is available as the non-conjugated format. GSTA1, a member of the glutathione S-transferase (GST) family, plays a crucial role in detoxifying xenobiotics, protecting cells from damage caused by toxic substances. GSTA1 is predominantly expressed in liver, lung, and kidney tissues, where GSTA1 helps mitigate the effects of environmental toxins and oxidative stress. GSTA1 is involved in conjugating harmful compounds, facilitating their excretion and reducing cellular toxicity. GSTA1′s structure includes a C-terminal domain contributing to the hydrophobic substrate-binding site, essential for enzymatic function, although GSTA1 does not directly bind glutathione. This structural feature allows GSTA1 to effectively interact with various substrates, enhancing GSTA1′s protective role in cellular defense mechanisms. The oncoprotein Myb transcriptionally upregulates GSTM1, another isoform of the GST family, highlighting the interconnectedness of these proteins in response to cellular stressors.

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GSTA1 Antibody (R-14) References:

1. Mechanisms of aflatoxin B1 lung tumorigenesis.  |  Massey, TE., et al. 2000. Exp Lung Res. 26: 673-83. PMID: 11195464
2. Multiple isoforms of mitochondrial glutathione S-transferases and their differential induction under oxidative stress.  |  Raza, H., et al. 2002. Biochem J. 366: 45-55. PMID: 12020353
3. Sulforaphane and its glutathione conjugate but not sulforaphane nitrile induce UDP-glucuronosyl transferase (UGT1A1) and glutathione transferase (GSTA1) in cultured cells.  |  Basten, GP., et al. 2002. Carcinogenesis. 23: 1399-404. PMID: 12151360
4. Regulation of the gene encoding glutathione S-transferase M1 (GSTM1) by the Myb oncoprotein.  |  Bartley, PA., et al. 2003. Oncogene. 22: 7570-5. PMID: 14576818
5. The contribution of the C-terminal sequence to the catalytic activity of GST2, a human alpha-class glutathione transferase.  |  Board, PG. and Mannervik, B. 1991. Biochem J. 275 (Pt 1): 171-4. PMID: 2018473
6. Common variants of GSTP1, GSTA1, and TGFβ1 are associated with the risk of radiation-induced fibrosis in breast cancer patients.  |  Terrazzino, S., et al. 2012. Int J Radiat Oncol Biol Phys. 83: 504-11. PMID: 22079734
7. Common polymorphisms in GSTM1, GSTT1, GSTP1, GSTA1 and susceptibility to colorectal cancer in the Central European population.  |  Hezova, R., et al. 2012. Eur J Med Res. 17: 17. PMID: 22697302
8. Effect of genetic variants GSTA1 and CYP39A1 and age on busulfan clearance in pediatric patients undergoing hematopoietic stem cell transplantation.  |  ten Brink, MH., et al. 2013. Pharmacogenomics. 14: 1683-90. PMID: 24192117
9. Review of the Pharmacokinetics and Pharmacodynamics of Intravenous Busulfan in Paediatric Patients.  |  Lawson, R., et al. 2021. Clin Pharmacokinet. 60: 17-51. PMID: 33128207
10. Structure determination and refinement of human alpha class glutathione transferase A1-1, and a comparison with the Mu and Pi class enzymes.  |  Sinning, I., et al. 1993. J Mol Biol. 232: 192-212. PMID: 8331657
11. Structural analysis of human alpha-class glutathione transferase A1-1 in the apo-form and in complexes with ethacrynic acid and its glutathione conjugate.  |  Cameron, AD., et al. 1995. Structure. 3: 717-27. PMID: 8591048
12. Increased levels of glutathione S transferases and appearance of novel alpha class isoenzymes in kidneys of mice exposed to mercuric chloride. I. Biochemical and immunohistochemical studies.  |  McGuire, S., et al. 1997. Nephron. 77: 452-60. PMID: 9434069

The preferred GSTA1/2/5 antibody is GSTA1/2/5 Antibody (E-6): sc-398714.