GSTA1/2/5 Antibody (E-6): sc-398714

GSTA1/2/5 Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects GSTA1/2/5 protein of mouse, GSTA1/3/5 of human origin, and GSTA2/5 of rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GSTA1/2/5 (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GSTA1/2/5 protein is a member of the glutathione S-transferase (GST) family, which plays a crucial role in detoxifying harmful compounds, including carcinogens and environmental toxins, thereby protecting cells from oxidative stress and damage. Specifically, the GSTA family, which includes GSTA1, GSTA2, GSTA3, and GSTA5, consists of highly homologous proteins that localize primarily in the cytoplasm and are predominantly expressed in the liver. These proteins exhibit peroxidase activity, which is vital for neutralizing reactive oxygen species and peroxidation by-products, and may also be involved in the metabolism of certain anti-cancer drugs, highlighting their importance in both cellular protection and therapeutic efficacy.

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GSTA1/2/5 Antibody (E-6) References:

1. Human glutathione transferase A3-3, a highly efficient catalyst of double-bond isomerization in the biosynthetic pathway of steroid hormones.  |  Johansson, AS. and Mannervik, B. 2001. J Biol Chem. 276: 33061-5. PMID: 11418619
2. Multiple isoforms of mitochondrial glutathione S-transferases and their differential induction under oxidative stress.  |  Raza, H., et al. 2002. Biochem J. 366: 45-55. PMID: 12020353
3. The human glutathione transferase alpha locus: genomic organization of the gene cluster and functional characterization of the genetic polymorphism in the hGSTA1 promoter.  |  Morel, F., et al. 2002. Pharmacogenetics. 12: 277-86. PMID: 12042665
4. Crystal structure of human glutathione S-transferase A3-3 and mechanistic implications for its high steroid isomerase activity.  |  Gu, Y., et al. 2004. Biochemistry. 43: 15673-9. PMID: 15595823
5. Structural basis for featuring of steroid isomerase activity in alpha class glutathione transferases.  |  Tars, K., et al. 2010. J Mol Biol. 397: 332-40. PMID: 20083122
6. The contribution of the C-terminal sequence to the catalytic activity of GST2, a human alpha-class glutathione transferase.  |  Board, PG. and Mannervik, B. 1991. Biochem J. 275 (Pt 1): 171-4. PMID: 2018473
7. Protective Effects of PEP-1-GSTA2 Protein in Hippocampal Neuronal Cell Damage Induced by Oxidative Stress.  |  Choi, YJ., et al. 2023. Int J Mol Sci. 24: PMID: 36769090
8. GSTA1/CTNNB1 axis facilitates sorafenib resistance via suppressing ferroptosis in hepatocellular carcinoma.  |  Ma, S., et al. 2024. Pharmacol Res. 210: 107490. PMID: 39510148
9. Structure determination and refinement of human alpha class glutathione transferase A1-1, and a comparison with the Mu and Pi class enzymes.  |  Sinning, I., et al. 1993. J Mol Biol. 232: 192-212. PMID: 8331657
10. Structural analysis of human alpha-class glutathione transferase A1-1 in the apo-form and in complexes with ethacrynic acid and its glutathione conjugate.  |  Cameron, AD., et al. 1995. Structure. 3: 717-27. PMID: 8591048
11. Increased levels of glutathione S transferases and appearance of novel alpha class isoenzymes in kidneys of mice exposed to mercuric chloride. I. Biochemical and immunohistochemical studies.  |  McGuire, S., et al. 1997. Nephron. 77: 452-60. PMID: 9434069
12. Identification of cDNAs encoding two human alpha class glutathione transferases (GSTA3 and GSTA4) and the heterologous expression of GSTA4-4.  |  Board, PG. 1998. Biochem J. 330 (Pt 2): 827-31. PMID: 9480897

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GSTA1/2/5 Antibody (E-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GSTA1/2/5 Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GSTA1/2/5 Antibody (E-6) Specifications

Alternate Product Name: GSTA1/2/5

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 189-217

Epitope Target: GSTA2

Epitope Species: mouse

Product Reactivity/Detection: GSTA1, GSTA3 and GSTA5 of human origin, GSTA1, GSTA2 and GSTA5 of mouse origin, and GSTA2 and GSTA5 of rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GSTA1/2/5 Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin