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GST Antibody (3D4): sc-57753

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Datasheets
  • GST Antibody (3D4) is a mouse monoclonal IgG1, cited in 15 publications, provided at 100 µg/ml
  • raised against GST of S. japonicum origin
  • recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin of Schistosoma japonicum origin by WB, IP and IF
  • See GST (B-14): sc-138 for GST antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GST Antibody (3D4). This work is in progress.
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GST Antibody (3D4) is a mouse monoclonal IgG1 antibody that detects glutathione-S-transferase (GST) in Schistosoma japonicum through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-GST antibody (3D4) is particularly valuable in research involving protein expression and purification, as GST serves as a widely used tag for recombinant proteins, facilitating isolation and characterization. GST protein plays a crucial role in detoxification processes within cells by catalyzing the conjugation of glutathione to various substrates, which is essential for the metabolism of potentially harmful compounds. This function is significant not only for cellular health but also for understanding how parasites like Schistosoma japonicum manage to survive in host organisms, making GST a target of interest in parasitology and drug development. GST′s ability to form stable fusion proteins with other proteins enhances the study of protein interactions and functions, providing insights into molecular mechanisms underlying various biological processes. GST monoclonal antibody (3D4) serves as an indispensable tool for researchers exploring protein dynamics in both basic and applied sciences.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

GST Antibody (3D4)

sc-57753
100 µg/ml
$322.00

what is recommended amount for IP?

Asked by: mac1982
Thank you for your question. To 1 ml of the above cell lysate, or approximately 100–1000 µg of total cellular protein, add 10 µg of primary antibody agarose conjugate (i.e., 5 µl volume of packed beads). You can find our recommended protocol for immunoprecipitation by following this link: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What is the total volume provided of GST (3D4): sc-57753 monoclonal antibody?

Asked by: Trav11
Thank you for your question. This antibody is provided as 100 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2017-03-07
  • y_2026, m_5, d_17, h_13CST
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Rated 5 out of 5 by from Published WB data using the following: 293T cellsPublished WB data using the following: 293T cells, HeLa cells, A431 cells, SW480 cells, RKO cells, SaOs cells, and MG63 cells. -SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from NiceNice, publishable WB data with 3D4. -SCBT Publication Review
Date published: 2015-03-31
Rated 5 out of 5 by from Strong band observed in Western blot analysisStrong band observed in Western blot analysis of recombinant GST protein. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Published data using 293T cells for Western blotPublished data using 293T cells for Western blot. -SCBT Publication Review
Date published: 2015-02-24
Rated 5 out of 5 by from Great western blot results when with HumanGreat western blot results when with Human embryonic fibroblast (HE) cells. -SCBT Publication Review
Date published: 2013-03-20
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GST Antibody (3D4) is rated 5.0 out of 5 by 5.
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