GSS Antibody (H-7): sc-166882

GSS Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects GSS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-GSS antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GSS protein, or Glutathione synthetase, is a crucial enzyme composed of 474 amino acids, encoded by a gene located on human chromosome 20q11.2. GSS plays a vital role in glutathione synthesis, a key antioxidant that protects cells from oxidative stress and maintains cellular redox balance. GSS (H-7) antibody targets proteins primarily located in the cytoplasm of hemocytes and nucleated cells, including those in the brain, where GSS functions as a homodimer. This enzyme catalyzes the ATP-dependent conversion of γ-glutamylcysteine and glycine into glutathione, highlighting its importance in cellular defense mechanisms. Dysregulation or deficiency of GSS can lead to serious health issues, such as the inherited autosomal recessive disorder 5-oxoprolinuria, which is characterized by central nervous system damage, hemolytic anemia, and metabolic acidosis due to impaired glutathione synthesis. Additionally, various factors, including partial hepatectomy and exposure to certain chemicals, can upregulate GSS expression, thereby increasing glutathione levels and enhancing cellular protection against oxidative damage.

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GSS Antibody (H-7) References:

1. Molecular basis of glutathione synthetase deficiency and a rare gene permutation event.  |  Polekhina, G., et al. 1999. EMBO J. 18: 3204-13. PMID: 10369661
2. Inducers of gamma-glutamylcysteine synthetase and their effects on glutathione synthetase expression.  |  Huang, ZA., et al. 2000. Biochim Biophys Acta. 1493: 48-55. PMID: 10978506
3. Gamma-glutamylcysteine synthetase-glutathione synthetase: domain structure and identification of residues important in substrate and glutathione binding.  |  Janowiak, BE., et al. 2006. Biochemistry. 45: 10461-73. PMID: 16939198
4. Glutathione synthetase promotes the reduction of arsenate via arsenolysis of glutathione.  |  Németi, B., et al. 2012. Biochimie. 94: 1327-33. PMID: 22426003
5. Enzymatic Production of Glutathione by Bifunctional γ-Glutamylcysteine Synthetase/Glutathione Synthetase Coupled with In Vitro Acetate Kinase-Based ATP Generation.  |  Jiang, Y., et al. 2016. Appl Biochem Biotechnol. 180: 1446-1455. PMID: 27380420
6. High-throughput screening suggests glutathione synthetase as an anti-tumor target of polydatin using human proteome chip.  |  Chen, P., et al. 2020. Int J Biol Macromol. 161: 1230-1239. PMID: 32544581
7. Gas Chromatography Mass Spectrometry Aided Diagnosis of Glutathione Synthetase Deficiency.  |  Kaur, P., et al. 2022. Lab Med. 53: e59-e61. PMID: 34791353
8. Uric acid effects on glutathione metabolism estimated by induction of glutamate-cysteine ligase, glutathione reductase and glutathione synthetase in mouse J744A.1 macrophage cell line.  |  Vankova, D., et al. 2022. Folia Med (Plovdiv). 64: 762-769. PMID: 36876528
9. Glutathione synthetase from Hydra vulgaris: Molecular cloning, overexpression, purification and partial characterization.  |  Perween, N., et al. 2023. Protein Expr Purif. 208-209: 106292. PMID: 37127055
10. Sequencing and expression of a cDNA for human glutathione synthetase.  |  Gali, RR. and Board, PG. 1995. Biochem J. 310 (Pt 1): 353-8. PMID: 7646467
11. The gene encoding human glutathione synthetase (GSS) maps to the long arm of chromosome 20 at band 11.2.  |  Webb, GC., et al. 1995. Genomics. 30: 617-9. PMID: 8825653
12. Mutations in the glutathione synthetase gene cause 5-oxoprolinuria.  |  Shi, ZZ., et al. 1996. Nat Genet. 14: 361-5. PMID: 8896573

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GSS Antibody (H-7) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-GSS Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GSS Antibody (H-7) Specifications

Alternate Product Name: GSS

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 81-380

Epitope Target: GSS

Epitope Species: human

Product Reactivity/Detection: GSS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for GSS Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin