Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GSDMDC1 CRISPR/Cas9 KO Plasmid (r): sc-437380
- Target species: rat
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSDMDC1 CRISPR/Cas9 Knockout (KO) Plasmid (r) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GSDMDC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GSDMDC1 Antibody (H-11): sc-393581
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSDMDC1 CRISPR/Cas9 KO Plasmid (r) | sc-437380 | 20 µg | $397.00 |
Overview
Rat GSDMDC1 encodes a gasdermin domain–containing protein linked to membrane remodeling and regulated cell death programs, processes that shape innate immune signaling and tissue homeostasis. Gasdermin family members are commonly positioned downstream of inflammatory protease cascades and can influence pore formation, ion flux, and release of inflammatory mediators, thereby intersecting with pathways such as inflammasome signaling and stress-induced cytotoxicity. Although GSDMDC1 remains less extensively characterized than canonical gasdermins, its domain architecture suggests roles in epithelial integrity and injury responses. Dysregulation of gasdermin-associated pathways has been connected to inflammatory and degenerative phenotypes, making GSDMDC1 a useful target for mechanistic studies in disease-relevant rat models.
GSDMDC1 CRISPR/Cas9 KO Plasmid (r) is a pool of plasmids designed for targeted disruption of the gene in rat cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GSDMDC1 protein expression.
This CRISPR knockout system enables efficient generation of -deficient cell models for investigation of GSDMDC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting exon(s) critical for GSDMDC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GSDMDC1 CRISPR/Cas9 KO Plasmid (r) and GSDMDC1 CRISPR/Cas9 KO Plasmid (r2) target distinct sites within the locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GSDMDC1 HDR Plasmid (r) and GSDMDC1 HDR Plasmid (r2) contain a puromycin resistance cassette and an RFP reporter flanked by homology arms to support homology-directed repair at defined target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.