GRO1 CRISPR/Cas9 KO Plasmid (m): sc-420697
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRO1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GRO1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GRO1 Antibody (S-19): sc-80516
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRO1 CRISPR/Cas9 KO Plasmid (m) | sc-420697 | 20 µg | $397.00 |
Overview
Mouse Cxcl1 encodes GRO1, a secreted CXC chemokine that signals primarily through CXCR2 to drive chemotaxis and activation of neutrophils and other myeloid populations. GRO1 participates in innate immune signaling, amplifies inflammatory cytokine networks, and contributes to endothelial activation and leukocyte extravasation through NF-κB- and MAPK-linked pathways. Dysregulated CXCL1/GRO1 expression is associated with inflammatory tissue injury, altered host defense, and microenvironmental changes relevant to infection and cancer biology. In experimental systems, Cxcl1 is widely used to probe chemokine gradients, leukocyte trafficking, and inflammation-driven remodeling.
GRO1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cxcl1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cxcl1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cxcl1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GRO1 protein expression.
This CRISPR knockout system enables efficient generation of Cxcl1-deficient cell models for investigation of GRO1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cxcl1 exon(s) critical for GRO1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cxcl1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GRO1 CRISPR/Cas9 KO Plasmid (m) and GRO1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cxcl1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GRO1 HDR Plasmid (m) and GRO1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cxcl1 homology arms to support homology-directed repair at defined Cxcl1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.