GRK 5 Antibody (D-9): sc-518005

GRK 5 Antibody (D-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects G protein-coupled receptor kinase 5 (GRK 5) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GRK 5 (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GRK 5 plays a crucial role in regulating G protein-coupled receptor signaling, particularly in agonist-mediated desensitization, which maintains cellular responsiveness to external signals. This desensitization occurs through phosphorylation of activated receptors, primarily mediated by G protein-coupled receptor kinases (GRKs), including GRK 5 . GRK 5 specifically phosphorylates receptors in their active state, ensuring signaling pathways can be finely tuned, preventing overstimulation and allowing cells to adapt to prolonged exposure to agonists. Understanding GRK 5 function is vital, as dysregulation can lead to various pathophysiological conditions, including heart disease and neurological disorders.

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GRK 5 Antibody (D-9) References:

1. The receptor kinase family: primary structure of rhodopsin kinase reveals similarities to the beta-adrenergic receptor kinase.  |  Lorenz, W., et al. 1991. Proc Natl Acad Sci U S A. 88: 8715-9. PMID: 1656454
2. Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family.  |  Benovic, JL., et al. 1991. J Biol Chem. 266: 14939-46. PMID: 1869533
3. Turning off the signal: desensitization of beta-adrenergic receptor function.  |  Hausdorff, WP., et al. 1990. FASEB J. 4: 2881-9. PMID: 2165947
4. The regulator of G protein signaling (RGS) domain of G protein-coupled receptor kinase 5 (GRK5) regulates plasma membrane localization and function.  |  Xu, H., et al. 2014. Mol Biol Cell. 25: 2105-15. PMID: 24807909
5. GRK5 - A Functional Bridge Between Cardiovascular and Neurodegenerative Disorders.  |  Hendrickx, JO., et al. 2018. Front Pharmacol. 9: 1484. PMID: 30618771
6. GRK5 is a regulator of fibroblast activation and cardiac fibrosis.  |  Eguchi, A., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33500351
7. G protein-coupled receptor kinase 5 (GRK5) contributes to impaired cardiac function and immune cell recruitment in post-ischemic heart failure.  |  de Lucia, C., et al. 2022. Cardiovasc Res. 118: 169-183. PMID: 33560342
8. Identification, purification, and characterization of GRK5, a member of the family of G protein-coupled receptor kinases.  |  Premont, RT., et al. 1994. J Biol Chem. 269: 6832-41. PMID: 8120045
9. An approach to the study of G-protein-coupled receptor kinases: an in vitro-purified membrane assay reveals differential receptor specificity and regulation by G beta gamma subunits.  |  Pei, G., et al. 1994. Proc Natl Acad Sci U S A. 91: 3633-6. PMID: 8170959
10. Functionally active targeting domain of the beta-adrenergic receptor kinase: an inhibitor of G beta gamma-mediated stimulation of type II adenylyl cyclase.  |  Inglese, J., et al. 1994. Proc Natl Acad Sci U S A. 91: 3637-41. PMID: 8170960
11. Structure and mechanism of the G protein-coupled receptor kinases.  |  Inglese, J., et al. 1993. J Biol Chem. 268: 23735-8. PMID: 8226899
12. Structural basis for receptor subtype-specific regulation revealed by a chimeric beta 3/beta 2-adrenergic receptor.  |  Liggett, SB., et al. 1993. Proc Natl Acad Sci U S A. 90: 3665-9. PMID: 8386380

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GRK 5 Antibody (D-9) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-GRK 5 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GRK 5 Antibody (D-9) Specifications

Alternate Product Name: GRK5

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 94-157

Epitope Target: GRK 5

Epitope Species: human

Product Reactivity/Detection: GRK 5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for GRK 5 Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin