Grim19 Antibody (F-10): sc-365978

Grim19 Antibody (F-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Grim19 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Grim19 antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Grim19, also known as gene associated with retinoic-interferon-induced mortality 19 protein, plays a crucial role in cellular processes, particularly in apoptosis regulation. As a member of mitochondrial complex I, Grim19 (F-10) antibody recognizes a protein essential for complex assembly and electron transfer activity, which is vital for cellular respiration and energy production. Through facilitating electron transfer from NADH to the respiratory chain, Grim19 supports mitochondrial function and participates in the interferon/all-trans-retinoic acid (IFN/RA) cell death pathway, highlighting a dual role in energy metabolism and apoptosis. Grim19′s ability to move from mitochondrion to nucleus upon IFN/RA treatment underscores significance in cellular signaling and potential as a therapeutic target. This multifaceted functionality positions Grim19 as a promising candidate for research in cancer biology and drug development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Grim19 Antibody (F-10) References:

1. Identification of GRIM-19, a novel cell death-regulatory gene induced by the interferon-beta and retinoic acid combination, using a genetic approach.  |  Angell, JE., et al. 2000. J Biol Chem. 275: 33416-26. PMID: 10924506
2. GRIM-19, a death-regulatory gene product, suppresses Stat3 activity via functional interaction.  |  Lufei, C., et al. 2003. EMBO J. 22: 1325-35. PMID: 12628925
3. The cell death regulator GRIM-19 is an inhibitor of signal transducer and activator of transcription 3.  |  Zhang, J., et al. 2003. Proc Natl Acad Sci U S A. 100: 9342-7. PMID: 12867595
4. Fine-mapping of the spinal muscular atrophy locus to a region flanked by MAP1B and D5S6.  |  Brzustowicz, LM., et al. 1992. Genomics. 13: 991-8. PMID: 1505990
5. The GRIMs: a new interface between cell death regulation and interferon/retinoid induced growth suppression.  |  Kalvakolanu, DV. 2004. Cytokine Growth Factor Rev. 15: 169-94. PMID: 15110800
6. GRIM-19, a cell death regulatory protein, is essential for assembly and function of mitochondrial complex I.  |  Huang, G., et al. 2004. Mol Cell Biol. 24: 8447-56. PMID: 15367666
7. Somatic and germline mutation in GRIM-19, a dual function gene involved in mitochondrial metabolism and cell death, is linked to mitochondrion-rich (Hurthle cell) tumours of the thyroid.  |  Máximo, V., et al. 2005. Br J Cancer. 92: 1892-8. PMID: 15841082
8. A proteomic analysis reveals the loss of expression of the cell death regulatory gene GRIM-19 in human renal cell carcinomas.  |  Alchanati, I., et al. 2006. Oncogene. 25: 7138-47. PMID: 16732315
9. Heterogeneity of GRIM-19 Expression in the Adult Mouse Brain.  |  Hwang, SN., et al. 2019. Cell Mol Neurobiol. 39: 935-951. PMID: 31111264
10. Upregulation of GRIM-19 augments the sensitivity of prostate cancer cells to docetaxel by targeting Rad23b.  |  Lin, H., et al. 2020. Clin Exp Pharmacol Physiol. 47: 76-84. PMID: 31531888
11. GRIM-19 inhibits proliferation and induces apoptosis in a p53-dependent manner in colorectal cancer cells through the SIRT7/PCAF/MDM2 axis.  |  Wang, D., et al. 2021. Exp Cell Res. 407: 112799. PMID: 34461110
12. Grim-19 plays a key role in mitochondrial steroidogenic acute regulatory protein stability and ligand-binding properties in Leydig cells.  |  Qu, H., et al. 2022. J Biol Chem. 298: 102671. PMID: 36334625

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Grim19 Antibody (F-10) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Grim19 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Grim19 Antibody (F-10) Specifications

Alternate Product Name: Grim19

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 62-132

Epitope Target: Grim19

Epitope Species: human

Product Reactivity/Detection: Grim19

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Grim19 Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin