GRF-1 Antibody (C-4): sc-390997
- GRF-1 Antibody (C-4) is a mouse monoclonal IgG1 κ GRF-1 antibody provided at 200 µg/ml
- raised against amino acids 881-1080 mapping within an internal region of GRF-1 of human origin
- GRF-1 Antibody (C-4) is recommended for detection of GRF-1 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GRF-1 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GRF-1 Antibody (C-4) for WB applications. These reagents are now offered in bundles with GRF-1 Antibody (C-4) (see ordering information below).
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GRF-1 Antibody (C-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects GRF-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GRF-1 (C-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GRF-1, also known as glucocorticoid receptor DNA-binding factor 1 or p190RhoGAP, plays a crucial role in regulating gene expression by binding to the promoter region of the glucocorticoid receptor gene, thereby repressing its expression. This repression down-regulates glucocorticoid receptor signaling, which is essential for mediating actin cytoskeletal rearrangements and cell cycle progression. GRF-1′s function is further underscored by involvement in oligodendrocyte differentiation, a process notably disrupted in malignant glioma tumors, suggesting GRF-1 may act as a tumor suppressor. Additionally, GRF-1 undergoes post-translational modifications, including ubiquitination, which regulates activity and stability by controlling protein degradation. GRF-1 expression is modulated by glucocorticoids, and exists in two isoforms due to alternative splicing, highlighting regulatory mechanism complexity and potential implications in various physiological and pathological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GRF-1 Antibody (C-4) References:
- Role of p190RhoGAP in beta 2 integrin regulation of RhoA in human neutrophils. | Dib, K., et al. 2001. J Immunol. 166: 6311-22. PMID: 11342655
- p190RhoGAP is cell cycle regulated and affects cytokinesis. | Su, L., et al. 2003. J Cell Biol. 163: 571-82. PMID: 14610059
- Adhesion-dependent regulation of p190RhoGAP in the developing brain by the Abl-related gene tyrosine kinase. | Hernández, SE., et al. 2004. Curr Biol. 14: 691-6. PMID: 15084284
- Suppression of RhoA activity by focal adhesion kinase-induced activation of p190RhoGAP: role in regulation of endothelial permeability. | Holinstat, M., et al. 2006. J Biol Chem. 281: 2296-305. PMID: 16308318
- PTP-PEST couples membrane protrusion and tail retraction via VAV2 and p190RhoGAP. | Sastry, SK., et al. 2006. J Biol Chem. 281: 11627-36. PMID: 16513648
- Inactivation of Rho GTPases by p190 RhoGAP reduces human pancreatic cancer cell invasion and metastasis. | Kusama, T., et al. 2006. Cancer Sci. 97: 848-53. PMID: 16776779
- p120-catenin and p190RhoGAP regulate cell-cell adhesion by coordinating antagonism between Rac and Rho. | Wildenberg, GA., et al. 2006. Cell. 127: 1027-39. PMID: 17129786
- The Abl-related gene tyrosine kinase acts through p190RhoGAP to inhibit actomyosin contractility and regulate focal adhesion dynamics upon adhesion to fibronectin. | Peacock, JG., et al. 2007. Mol Biol Cell. 18: 3860-72. PMID: 17652459
- Inhibition of Rho via Arg and p190RhoGAP in the postnatal mouse hippocampus regulates dendritic spine maturation, synapse and dendrite stability, and behavior. | Sfakianos, MK., et al. 2007. J Neurosci. 27: 10982-92. PMID: 17928439
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRF-1 Antibody (C-4) | sc-390997 | 200 µg/ml | $316.00 | |||
GRF-1 Antibody (C-4): m-IgG Fc BP-HRP Bundle | sc-530330 | 200 µg Ab; 10 µg BP | $354.00 | |||
GRF-1 Antibody (C-4): m-IgGκ BP-HRP Bundle | sc-523769 | 200 µg Ab, 40 µg BP | $354.00 | |||
GRF-1 Antibody (C-4) AC | sc-390997 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GRF-1 Antibody (C-4) HRP | sc-390997 HRP | 200 µg/ml | $316.00 | |||
GRF-1 Antibody (C-4) FITC | sc-390997 FITC | 200 µg/ml | $330.00 | |||
GRF-1 Antibody (C-4) PE | sc-390997 PE | 200 µg/ml | $343.00 | |||
GRF-1 Antibody (C-4) Alexa Fluor® 488 | sc-390997 AF488 | 200 µg/ml | $357.00 | |||
GRF-1 Antibody (C-4) Alexa Fluor® 546 | sc-390997 AF546 | 200 µg/ml | $357.00 | |||
GRF-1 Antibody (C-4) Alexa Fluor® 594 | sc-390997 AF594 | 200 µg/ml | $357.00 | |||
GRF-1 Antibody (C-4) Alexa Fluor® 647 | sc-390997 AF647 | 200 µg/ml | $357.00 | |||
GRF-1 Antibody (C-4) Alexa Fluor® 680 | sc-390997 AF680 | 200 µg/ml | $357.00 | |||
GRF-1 Antibody (C-4) Alexa Fluor® 790 | sc-390997 AF790 | 200 µg/ml | $357.00 |