GRB2 Antibody (H-9): sc-137056

GRB2 Antibody (H-9) is a mouse monoclonal IgG1 antibody that detects GRB2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GRB2 plays a crucial role in cellular signaling pathways, particularly in the regulation of growth factor receptor signaling, which is essential for cell proliferation and differentiation. GRB2 is primarily located in the cytoplasm and is recruited to the plasma membrane upon activation of receptor tyrosine kinases, such as the epidermal growth factor receptor (EGFR). GRB2 localization facilitates the assembly of signaling complexes that propagate downstream signaling cascades, ultimately influencing cellular responses to external stimuli. GRB2 interacts with several key proteins, including the guanine nucleotide exchange factor SOS1, which is critical for activating Ras proteins, thereby linking receptor activation to the Ras signaling pathway. GRB2′s ability to form complexes with these proteins underscores its importance in mediating cellular responses and highlights its potential as a target for therapeutic interventions in diseases characterized by dysregulated signaling pathways.

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GRB2 Antibody (H-9) References:

1. Grb2 and Nck act cooperatively to promote actin-based motility of vaccinia virus.  |  Scaplehorn, N., et al. 2002. Curr Biol. 12: 740-5. PMID: 12007418
2. Grb2 regulates Stat3 activation negatively in epidermal growth factor signalling.  |  Zhang, T., et al. 2003. Biochem J. 376: 457-64. PMID: 14498832
3. grb2 heterozygosity rescues embryonic lethality but not tumorigenesis in pten+/- mice.  |  Cully, M., et al. 2004. Proc Natl Acad Sci U S A. 101: 15358-63. PMID: 15492213
4. Grb2 adaptor undergoes conformational change upon dimerization.  |  McDonald, CB., et al. 2008. Arch Biochem Biophys. 475: 25-35. PMID: 18442468
5. Grb2 controls phosphorylation of FGFR2 by inhibiting receptor kinase and Shp2 phosphatase activity.  |  Ahmed, Z., et al. 2013. J Cell Biol. 200: 493-504. PMID: 23420874
6. Grb2 dimer interacts with Coumarin through SH2 domains: A combined experimental and molecular modeling study.  |  Sanches, K., et al. 2019. Heliyon. 5: e02869. PMID: 31844748
7. The intramolecular allostery of GRB2 governing its interaction with SOS1 is modulated by phosphotyrosine ligands.  |  Kazemein Jasemi, NS., et al. 2021. Biochem J. 478: 2793-2809. PMID: 34232285
8. Evaluation of the relationship between miR-1271 and GRB2 gene in endometriosis.  |  Yarahmadi, G., et al. 2022. Eur J Obstet Gynecol Reprod Biol. 277: 12-15. PMID: 35970002
9. ERRFI1 exacerbates hepatic ischemia reperfusion injury by promoting hepatocyte apoptosis and ferroptosis in a GRB2-dependent manner.  |  Zhao, H. and Mao, H. 2024. Mol Med. 30: 82. PMID: 38862918
10. Signaling through Grb2/Ash-control of the Ras pathway and cytoskeleton.  |  Takenawa, T., et al. 1998. Curr Top Microbiol Immunol. 228: 325-42. PMID: 9401211

The preferred GRB2 antibody is GRB2 Antibody (C-7): sc-8034.