GRB2 Antibody (C-7) recommended for detection of GRB2 p25 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

GRB2 Antibody (C-7): sc-8034

GRB2 Antibody (C-7) is rated 4.7 out of 5 by 12.
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Datasheets

    • Anti-GRB2 Antibody (C-7) is a mouse monoclonal IgG1 (kappa light chain) GRB2 antibody provided at 200 µg/ml
    • raised against amino acids 54-164 mapping to a central domain of GRB2 of mouse origin
    • Anti-GRB2 Antibody (C-7) is recommended for detection of GRB2 p25 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-GRB2 Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GRB2 (C-7): sc-8034.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 4 out of 5 by from work well for WB I used this antibody for WB with recommended dilution ratio. And it worked well for all the samples with very clear and specific bands. My samples included both cell lines (MEF and SY5Y) and mice tissues (liver, lung and kidney).
Date published: 2018-03-15
Rated 4 out of 5 by from Good for WB I used it in mouse lymphocytes and worked well for WB.
Date published: 2018-02-14
Rated 5 out of 5 by from Sal The antibody work fine I hav tried with whole cell lysate from Jurkat T cells
Date published: 2017-10-17
Rated 4 out of 5 by from Great antibody in specificity It works well in WB at 1:400, C2C12 cells and has a good specificity.
Date published: 2017-08-17
Rated 5 out of 5 by from Excellent antibody We get pretty results in WB, with a strong, clean 25 kDa band and one weak additional band below.The background is clean. The working dilution is 1: 500.
Date published: 2017-06-03
Rated 5 out of 5 by from Worked well in WB It shows a great sensitivity and specificity in WB, I recommend this product.
Date published: 2017-05-13
Rated 5 out of 5 by from Good WB Used 1:1000 for Western blot in human tonsil tissue, got expected bands
Date published: 2017-04-22
Rated 5 out of 5 by from Outstanding WB and IF Great western data; strong, clear signal; also nice IF staining
Date published: 2017-01-27
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