GRASP55 Antibody (E-5): sc-271840

GRASP55 Antibody (E-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects GRASP55 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GRASP55 (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GRASP55, also known as Golgi reassembly stacking protein or p59, plays a crucial role in the structure and function of the Golgi apparatus, a vital organelle responsible for processing and packaging proteins for secretion. GRASP55 is primarily located in the medial-Golgi, where GRASP55 is essential for the stacking of Golgi cisternae, a process critical for maintaining the integrity and functionality of the Golgi structure. GRASP55 is highly homologous to GRASP65 and features two PDZ domains, which are important for protein-protein interactions. GRASP55 undergoes post-translational modifications, including myristoylation and palmitoylation, which are essential for membrane association and function. GRASP55 interacts with the novel coiled-coil protein golgin 45 and the GTP-bound form of Rab 2, forming a complex crucial for normal protein transport and Golgi architecture. Phosphorylation of GRASP55 by ERK2 during mitosis indicates involvement in the regulation of Golgi dynamics in response to cellular signaling pathways, highlighting importance in cellular processes.

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GRASP55 Antibody (E-5) References:

1. GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system.  |  Shorter, J., et al. 1999. EMBO J. 18: 4949-60. PMID: 10487747
2. Transmembrane transforming growth factor-alpha tethers to the PDZ domain-containing, Golgi membrane-associated protein p59/GRASP55.  |  Kuo, A., et al. 2000. EMBO J. 19: 6427-39. PMID: 11101516
3. Mitotic phosphorylation of Golgi reassembly stacking protein 55 by mitogen-activated protein kinase ERK2.  |  Jesch, SA., et al. 2001. Mol Biol Cell. 12: 1811-7. PMID: 11408587
4. A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic.  |  Short, B., et al. 2001. J Cell Biol. 155: 877-83. PMID: 11739401
5. Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus.  |  Barr, FA., et al. 2001. J Cell Biol. 155: 885-91. PMID: 11739402
6. GRASP55: A Multifunctional Protein.  |  Wu, H., et al. 2020. Curr Protein Pept Sci. 21: 544-552. PMID: 32067616
7. GRASP55 Is Dispensable for Normal Hematopoiesis but Necessary for Myc-Dependent Leukemic Growth.  |  Bailly, AL., et al. 2020. J Immunol. 204: 2685-2696. PMID: 32229537
8. Nonredundant Roles of GRASP55 and GRASP65 in the Golgi Apparatus and Beyond.  |  Zhang, X. and Wang, Y. 2020. Trends Biochem Sci. 45: 1065-1079. PMID: 32893104
9. An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.  |  Nüchel, J., et al. 2021. Mol Cell. 81: 3275-3293.e12. PMID: 34245671
10. GRASP55 regulates intra-Golgi localization of glycosylation enzymes to control glycosphingolipid biosynthesis.  |  Pothukuchi, P., et al. 2021. EMBO J. 40: e107766. PMID: 34516001
11. GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.  |  Ahat, E., et al. 2022. J Biol Chem. 298: 102219. PMID: 35780830
12. Identification and Characterization of GRASP55 O-GlcNAcylation.  |  Zhang, X. 2023. Methods Mol Biol. 2557: 743-753. PMID: 36512248

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GRASP55 Antibody (E-5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-GRASP55 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GRASP55 Antibody (E-5) Specifications

Alternate Product Name: GRASP55

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 11-29

Epitope Target: GRASP55

Epitope Species: human

Product Reactivity/Detection: GRASP55

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for GRASP55 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin