Grancalcin Antibody (H-11): sc-393681
- Grancalcin Antibody (H-11) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 1-65 mapping at the N-terminus of Grancalcin of mouse origin
- Grancalcin Antibody (H-11) is recommended for detection of Grancalcin of mouse origin by WB, IP, IF and ELISA
- Anti-Grancalcin Antibody (H-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Grancalcin Antibody (H-11) for WB applications. These reagents are now offered in bundles with Grancalcin Antibody (H-11) (see ordering information below).
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SEE ALSO...
Grancalcin Antibody (H-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Grancalcin in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Grancalcin antibody (H-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Grancalcin is a calcium-binding protein predominantly found in human neutrophils and is a member of the penta EF-hand (PEF) subfamily of EF-hand proteins, which includes calpain, sorcin, peflin, and ALG-2. Grancalcin′s structure is characterized by homodimer formation through unpaired EF5 hands, crucial for function. Upon calcium binding, Grancalcin undergoes significant conformational changes that expose hydrophobic amino acid residues, allowing interaction with hydrophobic surfaces, a process vital for cellular signaling and adhesion. Grancalcin interacts with L-plastin, a protein known for actin bundling activity, suggesting a role in regulating neutrophil adhesion and migration. This interaction emphasizes Grancalcin′s importance in immune responses, particularly during inflammation and infection where neutrophil function is critical. Grancalcin expression primarily occurs in bone marrow-derived cells, with high abundance in neutrophils and monocytes, while only trace amounts exist in lymphocytes, highlighting a specialized role in immune system function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Grancalcin Antibody (H-11) | sc-393681 | 200 µg/ml | $322.00 | |||
Grancalcin Antibody (H-11): m-IgG Fc BP-HRP Bundle | sc-530538 | 200 µg Ab; 10 µg BP | $361.00 | |||
Grancalcin Antibody (H-11): m-IgGκ BP-HRP Bundle | sc-524044 | 200 µg Ab, 40 µg BP | $361.00 | |||
Grancalcin Antibody (H-11): m-IgG1 BP-HRP Bundle | sc-544114 | 200 µg Ab; 20 µg BP | $361.00 | |||
Grancalcin Antibody (H-11) AC | sc-393681 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Grancalcin Antibody (H-11) HRP | sc-393681 HRP | 200 µg/ml | $322.00 | |||
Grancalcin Antibody (H-11) FITC | sc-393681 FITC | 200 µg/ml | $336.00 | |||
Grancalcin Antibody (H-11) PE | sc-393681 PE | 200 µg/ml | $349.00 | |||
Grancalcin Antibody (H-11) Alexa Fluor® 488 | sc-393681 AF488 | 200 µg/ml | $364.00 | |||
Grancalcin Antibody (H-11) Alexa Fluor® 546 | sc-393681 AF546 | 200 µg/ml | $364.00 | |||
Grancalcin Antibody (H-11) Alexa Fluor® 594 | sc-393681 AF594 | 200 µg/ml | $364.00 | |||
Grancalcin Antibody (H-11) Alexa Fluor® 647 | sc-393681 AF647 | 200 µg/ml | $364.00 | |||
Grancalcin Antibody (H-11) Alexa Fluor® 680 | sc-393681 AF680 | 200 µg/ml | $364.00 | |||
Grancalcin Antibody (H-11) Alexa Fluor® 790 | sc-393681 AF790 | 200 µg/ml | $364.00 |