Glutathione Peroxidase 5/GPX5 Antibody (D-3): sc-390092
- Glutathione Peroxidase 5/GPX5 Antibody (D-3) is a mouse monoclonal IgG2b κ Glutathione Peroxidase 5/GPX5 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 22-66 mapping near the N-terminus of GPx-5 of human origin
- Glutathione Peroxidase 5/GPX5 Antibody (D-3) is recommended for detection of GPx-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Glutathione Peroxidase 5/GPX5 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Glutathione Peroxidase 5/GPX5 Antibody (D-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with Glutathione Peroxidase 5/GPX5 Antibody (D-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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GPx-5 Antibody (D-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects GPx-5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GPx-5 Antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GPx-5 protein, also known as Epididymis-Specific Glutathione Peroxidase-Like Protein, plays a crucial role in the male reproductive system, particularly in protecting sperm from oxidative damage. Located in the acrosome of sperm, GPx-5 is unique among glutathione peroxidases as GPx-5 is selenium-independent, which allows for function in environments where selenium is limited. This localization is vital for maintaining sperm integrity and function during maturation in the epididymis, thereby contributing to male fertility. GPx-5 helps prevent premature acrosome reactions, which can lead to reduced sperm viability and fertility issues. Understanding GPx-5′s role in sperm maturation and protection against oxidative stress is essential for advancing reproductive health research and developing potential therapeutic strategies for male infertility.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Glutathione Peroxidase 5/GPX5 Antibody (D-3) References:
- Increased resistance of GPx-1 transgenic mice to tumor promoter-induced loss of glutathione peroxidase activity in skin. | Bilodeau, JF. and Mirault, ME. 1999. Int J Cancer. 80: 863-7. PMID: 10074919
- Inverse mRNA expression of the selenocysteine-containing proteins GI-GPx and SeP in colorectal adenomas compared with adjacent normal mucosa. | Mörk, H., et al. 2000. Nutr Cancer. 37: 108-16. PMID: 10965527
- Increased infarct size and exacerbated apoptosis in the glutathione peroxidase-1 (Gpx-1) knockout mouse brain in response to ischemia/reperfusion injury. | Crack, PJ., et al. 2001. J Neurochem. 78: 1389-99. PMID: 11579147
- p53-induced up-regulation of MnSOD and GPx but not catalase increases oxidative stress and apoptosis. | Hussain, SP., et al. 2004. Cancer Res. 64: 2350-6. PMID: 15059885
- Determinants of human plasma glutathione peroxidase (GPx-3) expression. | Bierl, C., et al. 2004. J Biol Chem. 279: 26839-45. PMID: 15096516
- GPx-1 modulates Akt and P70S6K phosphorylation and Gadd45 levels in MCF-7 cells. | Nasr, MA., et al. 2004. Free Radic Biol Med. 37: 187-95. PMID: 15203190
- Functional variants in the glutathione peroxidase-1 (GPx-1) gene are associated with increased intima-media thickness of carotid arteries and risk of macrovascular diseases in japanese type 2 diabetic patients. | Hamanishi, T., et al. 2004. Diabetes. 53: 2455-60. PMID: 15331559
- Glutathione peroxidase 2, the major cigarette smoke-inducible isoform of GPX in lungs, is regulated by Nrf2. | Singh, A., et al. 2006. Am J Respir Cell Mol Biol. 35: 639-50. PMID: 16794261
- SYVN1/GPX5 axis affects ischemia/reperfusion induced apoptosis of AC16 cells by regulating ROS generation. | Zhang, J., et al. 2021. Am J Transl Res. 13: 4055-4067. PMID: 34149998
- Expression and chromosomal mapping of mouse Gpx2 gene encoding the gastrointestinal form of glutathione peroxidase, GPX-GI. | Chu, FF., et al. 1997. Biomed Environ Sci. 10: 156-62. PMID: 9315306
- The PEA3 protein of the Ets oncogene family is a putative transcriptional modulator of the mouse epididymis-specific glutathione peroxidase gene gpx5. | Drevet, JR., et al. 1998. Mol Reprod Dev. 49: 131-40. PMID: 9444656
- The majority of human glutathione peroxidase type 5 (GPX5) transcripts are incorrectly spliced: implications for the role of GPX5 in the male reproductive tract. | Hall, L., et al. 1998. Biochem J. 333 (Pt 1): 5-9. PMID: 9639555
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glutathione Peroxidase 5/GPX5 Antibody (D-3) | sc-390092 | 200 µg/ml | $316.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-523399 | 200 µg Ab, 40 µg BP | $354.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3) AC | sc-390092 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3) HRP | sc-390092 HRP | 200 µg/ml | $316.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3) FITC | sc-390092 FITC | 200 µg/ml | $330.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3) PE | sc-390092 PE | 200 µg/ml | $343.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3) Alexa Fluor® 488 | sc-390092 AF488 | 200 µg/ml | $357.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3) Alexa Fluor® 546 | sc-390092 AF546 | 200 µg/ml | $357.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3) Alexa Fluor® 594 | sc-390092 AF594 | 200 µg/ml | $357.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3) Alexa Fluor® 647 | sc-390092 AF647 | 200 µg/ml | $357.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3) Alexa Fluor® 680 | sc-390092 AF680 | 200 µg/ml | $357.00 | |||
Glutathione Peroxidase 5/GPX5 Antibody (D-3) Alexa Fluor® 790 | sc-390092 AF790 | 200 µg/ml | $357.00 |