Glutathione Peroxidase 5/GPX5 Antibody (D-2): sc-376877

GPx-5 Antibody (D-2) is a mouse monoclonal IgG2a antibody that detects glutathione peroxidase 5 (GPx-5) in mouse and rat samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GPx-5 is a unique selenium-independent isoform of the glutathione peroxidase family, primarily localized in the acrosome of sperm cells, where GPx-5 plays a crucial role in protecting sperm from oxidative damage during maturation in the epididymis. This localization helps maintain sperm viability and function, which is essential for successful fertilization. GPx-5 presence in the acrosome suggests involvement in preventing premature acrosome reactions, thereby ensuring sperm are fully prepared for the fertilization process. With an ability to mitigate oxidative stress, GPx-5 is vital for male fertility, and GPx-5 dysregulation could potentially lead to reproductive issues. Anti-GPx-5 antibody (D-2) is an invaluable tool for researchers studying GPx-5′s role in reproductive biology and oxidative stress responses.

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Glutathione Peroxidase 5/GPX5 Antibody (D-2) References:

1. Selenium-independent epididymis-restricted glutathione peroxidase 5 protein (GPX5) can back up failing Se-dependent GPXs in mice subjected to selenium deficiency.  |  Vernet, P., et al. 1999. Mol Reprod Dev. 54: 362-70. PMID: 10542376
2. GPX5 orthologs of the mouse epididymis-restricted and sperm-bound selenium-independent glutathione peroxidase are not expressed with the same quantitative and spatial characteristics in large domestic animals.  |  Grignard, E., et al. 2005. Theriogenology. 64: 1016-33. PMID: 16054503
3. GPX5, the selenium-independent glutathione peroxidase-encoding single copy gene is differentially expressed in mouse epididymis.  |  Zhang, T., et al. 2008. Reprod Fertil Dev. 20: 615-25. PMID: 18577359
4. Epididymis seleno-independent glutathione peroxidase 5 maintains sperm DNA integrity in mice.  |  Chabory, E., et al. 2009. J Clin Invest. 119: 2074-85. PMID: 19546506
5. Epididymal specific, selenium-independent GPX5 protects cells from oxidative stress-induced lipid peroxidation and DNA mutation.  |  Taylor, A., et al. 2013. Hum Reprod. 28: 2332-42. PMID: 23696541
6. Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice.  |  Chu, C., et al. 2020. Asian J Androl. 22: 590-601. PMID: 32270769
7. Exploring novel function of Gpx5 antioxidant activity: Assisting epididymal cells secrete functional extracellular vesicles.  |  Li, L., et al. 2024. J Cell Physiol. 239: e31273. PMID: 38666419
8. Tissue and developmental distribution, dependence upon testicular factors and attachment to spermatozoa of GPX5, a murine epididymis-specific glutathione peroxidase.  |  Vernet, P., et al. 1997. Mol Reprod Dev. 47: 87-98. PMID: 9110319
9. The majority of human glutathione peroxidase type 5 (GPX5) transcripts are incorrectly spliced: implications for the role of GPX5 in the male reproductive tract.  |  Hall, L., et al. 1998. Biochem J. 333 (Pt 1): 5-9. PMID: 9639555
10. Expression of extracellular glutathione peroxidase type 5 (GPX5) in the rat male reproductive tract.  |  Williams, K., et al. 1998. Mol Hum Reprod. 4: 841-8. PMID: 9783843

The preferred Glutathione Peroxidase 1/2/GPX1/2 antibody is Glutathione Peroxidase 1/2/GPX1/2 Antibody (B-6): sc-133160.