Glutathione Peroxidase 4/GPX4 Antibody (D-3): sc-166437

GPx-4 Antibody (D-3) is a mouse monoclonal IgG2a antibody that detects glutathione peroxidase 4 (GPx-4) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GPx-4 is a unique member of the glutathione peroxidase family, notable for its ability to reduce lipid hydroperoxides and protect cells from oxidative damage, which is crucial for maintaining cellular integrity and function. Unlike other GPx isoforms, GPx-4 is primarily localized in the mitochondria and is essential for protecting sperm cells from oxidative stress during their maturation in the epididymis. This localization is significant because GPx-4 safeguards cellular components from reactive oxygen species, thereby preventing lipid peroxidation and ensuring proper cellular function. Additionally, GPx-4 forms high molecular weight oligomers, which may enhance protective capabilities against oxidative damage. Anti-GPx-4 antibody (D-3) serves as an invaluable tool for researchers studying oxidative stress responses and GPx-4′s role in various physiological and pathological processes.

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Glutathione Peroxidase 4/GPX4 Antibody (D-3) References:

1. Inactivation of the ferroptosis regulator Gpx4 triggers acute renal failure in mice.  |  Friedmann Angeli, JP., et al. 2014. Nat Cell Biol. 16: 1180-91. PMID: 25402683
2. GPX4 at the Crossroads of Lipid Homeostasis and Ferroptosis.  |  Forcina, GC. and Dixon, SJ. 2019. Proteomics. 19: e1800311. PMID: 30888116
3. The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.  |  Bersuker, K., et al. 2019. Nature. 575: 688-692. PMID: 31634900
4. Redox homeostasis maintained by GPX4 facilitates STING activation.  |  Jia, M., et al. 2020. Nat Immunol. 21: 727-735. PMID: 32541831
5. Contribution of ferroptosis and GPX4's dual functions to osteoarthritis progression.  |  Miao, Y., et al. 2022. EBioMedicine. 76: 103847. PMID: 35101656
6. Mechanical overloading induces GPX4-regulated chondrocyte ferroptosis in osteoarthritis via Piezo1 channel facilitated calcium influx.  |  Wang, S., et al. 2022. J Adv Res. 41: 63-75. PMID: 36328754
7. Creatine kinase B suppresses ferroptosis by phosphorylating GPX4 through a moonlighting function.  |  Wu, K., et al. 2023. Nat Cell Biol. 25: 714-725. PMID: 37156912
8. Ferroptosis surveillance independent of GPX4 and differentially regulated by sex hormones.  |  Liang, D., et al. 2023. Cell. 186: 2748-2764.e22. PMID: 37267948
9. GPX4 in cell death, autophagy, and disease.  |  Xie, Y., et al. 2023. Autophagy. 19: 2621-2638. PMID: 37272058
10. USP8-governed GPX4 homeostasis orchestrates ferroptosis and cancer immunotherapy.  |  Li, H., et al. 2024. Proc Natl Acad Sci U S A. 121: e2315541121. PMID: 38598341

The preferred Glutathione Peroxidase 4/GPX4 antibody is Glutathione Peroxidase 4/GPX4 Antibody (B-12): sc-166120.