GPNMB Antibody (D-9): sc-271415
- GPNMB Antibody (D-9) is a mouse monoclonal IgG1 κ GPNMB antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 38-158 mapping near the N-terminus of GPNMB of human origin
- GPNMB Antibody (D-9) is recommended for detection of GPNMB isoforms 1 and 2 of human origin by WB, IP, IF and ELISA
- Anti-GPNMB Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GPNMB Antibody (D-9) for WB applications. These reagents are now offered in bundles with GPNMB Antibody (D-9) (see ordering information below).
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GPNMB Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects GPNMB protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-GPNMB antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GPNMB, also known as hematopoietic growth factor inducible neurokinin-1 (HGFIN), is a single-pass type I transmembrane glycoprotein that plays a crucial role in cellular processes, particularly in melanoma. GPNMB is involved in melanogenesis regulation and shows higher expression in poorly metastatic melanoma cell lines, while remaining absent in highly metastatic variants. This differential expression suggests GPNMB may serve as a potential biomarker for melanoma progression and could be significant in understanding tumor metastasis mechanisms. GPNMB is also implicated in lymphohematopoietic stem cell maturation, highlighting significance beyond melanocyte biology. GPNMB monoclonal antibody (D-9) effectively detects this target across various applications, making this reagent invaluable for researchers studying melanoma and stem cell biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GPNMB Antibody (D-9) References:
- Mutations in genes encoding melanosomal proteins cause pigmentary glaucoma in DBA/2J mice. | Anderson, MG., et al. 2002. Nat Genet. 30: 81-5. PMID: 11743578
- Cloning and characterization of osteoactivin, a novel cDNA expressed in osteoblasts. | Safadi, FF., et al. 2001. J Cell Biochem. 84: 12-26. PMID: 11746512
- Hematopoietic growth factor inducible neurokinin-1 type: a transmembrane protein that is similar to neurokinin 1 interacts with substance P. | Bandari, PS., et al. 2003. Regul Pept. 111: 169-78. PMID: 12609765
- mRNA expression of the murine glycoprotein (transmembrane) nmb (Gpnmb) gene is linked to the developing retinal pigment epithelium and iris. | Bächner, D., et al. 2002. Brain Res Gene Expr Patterns. 1: 159-65. PMID: 12638126
- Osteoactivin expressed during cirrhosis development in rats fed a choline-deficient, L-amino acid-defined diet, accelerates motility of hepatoma cells. | Onaga, M., et al. 2003. J Hepatol. 39: 779-85. PMID: 14568261
- Cloning and characterization of the 5' flanking region of the HGFIN gene indicate a cooperative role among p53 and cytokine-mediated transcription factors: relevance to cell cycle regulation. | Metz, RL., et al. 2005. Cell Cycle. 4: 315-22. PMID: 15684612
- Expression of osteoactivin in rat and human liver and isolated rat liver cells. | Haralanova-Ilieva, B., et al. 2005. J Hepatol. 42: 565-72. PMID: 15763343
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPNMB Antibody (D-9) | sc-271415 | 200 µg/ml | $322.00 | |||
GPNMB Antibody (D-9): m-IgG Fc BP-HRP Bundle | sc-529179 | 200 µg Ab; 10 µg BP | $361.00 | |||
GPNMB Antibody (D-9): m-IgGκ BP-HRP Bundle | sc-521937 | 200 µg Ab, 40 µg BP | $361.00 | |||
GPNMB Antibody (D-9) AC | sc-271415 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GPNMB Antibody (D-9) HRP | sc-271415 HRP | 200 µg/ml | $322.00 | |||
GPNMB Antibody (D-9) FITC | sc-271415 FITC | 200 µg/ml | $336.00 | |||
GPNMB Antibody (D-9) PE | sc-271415 PE | 200 µg/ml | $349.00 | |||
GPNMB Antibody (D-9) Alexa Fluor® 488 | sc-271415 AF488 | 200 µg/ml | $364.00 | |||
GPNMB Antibody (D-9) Alexa Fluor® 546 | sc-271415 AF546 | 200 µg/ml | $364.00 | |||
GPNMB Antibody (D-9) Alexa Fluor® 594 | sc-271415 AF594 | 200 µg/ml | $364.00 | |||
GPNMB Antibody (D-9) Alexa Fluor® 647 | sc-271415 AF647 | 200 µg/ml | $364.00 | |||
GPNMB Antibody (D-9) Alexa Fluor® 680 | sc-271415 AF680 | 200 µg/ml | $364.00 | |||
GPNMB Antibody (D-9) Alexa Fluor® 790 | sc-271415 AF790 | 200 µg/ml | $364.00 |