GPD2 Antibody (D-12): sc-390830

GPD2 Antibody (D-12) is a mouse monoclonal IgM antibody that detects GPD2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-GPD2 antibody (D-12) is available as the non-conjugated form. GPD2, or glycerol-3-phosphate dehydrogenase 2, mitochondrial, is a 727 amino acid protein that plays a crucial role in cellular metabolism by facilitating the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate while reducing enzyme-bound flavin adenine dinucleotide (FAD). This enzymatic activity is vital for maintaining the balance of redox states within the cell, particularly in energy production and glucose metabolism. GPD2 is predominantly located on the outer surface of the inner mitochondrial membrane, where GPD2 collaborates with GPD1, a cytosolic NAD-linked glycerol-3-phosphate dehydrogenase, to form a glycerol phosphate shuttle. This shuttle is essential for the reoxidation of NADH generated during glycolysis, thereby linking carbohydrate metabolism to mitochondrial function. GPD2 is highly expressed in human pancreatic islets, where GPD2 is critical for the glucose-sensing function of pancreatic beta cells; decreased levels of GPD2 have been associated with impaired glucose-stimulated insulin release, a hallmark of noninsulin-dependent diabetes mellitus. GPD2 exists in two alternatively spliced isoforms and contains two EF-hand domains, mapping to human chromosome 2q24.1, underscoring GPD2′s importance in metabolic regulation and potential implications in metabolic disorders.

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GPD2 Antibody (D-12) References:

1. Functional analysis of two promoters for the human mitochondrial glycerol phosphate dehydrogenase gene.  |  Gong, Q., et al. 2000. J Biol Chem. 275: 38012-21. PMID: 10954707
2. Normal thyroid thermogenesis but reduced viability and adiposity in mice lacking the mitochondrial glycerol phosphate dehydrogenase.  |  Brown, LJ., et al. 2002. J Biol Chem. 277: 32892-8. PMID: 12093799
3. Lethal hypoglycemic ketosis and glyceroluria in mice lacking both the mitochondrial and the cytosolic glycerol phosphate dehydrogenases.  |  Brown, LJ., et al. 2002. J Biol Chem. 277: 32899-904. PMID: 12093800
4. Haploinsufficiency of the GPD2 gene in a patient with nonsyndromic mental retardation.  |  Daoud, H., et al. 2009. Hum Genet. 124: 649-58. PMID: 19011903
5. Intellectual disability and hemizygous GPD2 mutation.  |  Barge-Schaapveld, DQ., et al. 2013. Am J Med Genet A. 161A: 1044-50. PMID: 23554088
6. Esculetin Inhibits Cancer Cell Glycolysis by Binding Tumor PGK2, GPD2, and GPI.  |  Wu, ST., et al. 2020. Front Pharmacol. 11: 379. PMID: 32292350
7. SPARC Controls Migration and Invasion of Hepatocellular Carcinoma Cells Via Regulating GPD2-Mediated Mitochondrial Respiration.  |  Liu, L., et al. 2024. Biochem Genet. 62: 4518-4535. PMID: 38334876
8. Human mitochondrial glycerol phosphate dehydrogenase (GPDm) isozymes.  |  Shaw, MA., et al. 1982. Ann Hum Genet. 46: 11-23. PMID: 6808887
9. Mitochondrial glycerol-3-phosphate dehydrogenase. Cloning of an alternatively spliced human islet-cell cDNA, tissue distribution, physical mapping, and identification of a polymorphic genetic marker.  |  Ferrer, J., et al. 1996. Diabetes. 45: 262-6. PMID: 8549872
10. Normalization by insulin treatment of low mitochondrial glycerol phosphate dehydrogenase and pyruvate carboxylase in pancreatic islets of the GK rat.  |  MacDonald, MJ., et al. 1996. Diabetes. 45: 886-90. PMID: 8666138
11. Structural organization and mapping of the human mitochondrial glycerol phosphate dehydrogenase-encoding gene and pseudogene.  |  Brown, LJ., et al. 1996. Gene. 172: 309-12. PMID: 8682323
12. Mutation in the calcium-binding domain of the mitochondrial glycerophosphate dehydrogenase gene in a family of diabetic subjects.  |  Novials, A., et al. 1997. Biochem Biophys Res Commun. 231: 570-2. PMID: 9070847

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GPD2 Antibody (D-12) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-GPD2 Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GPD2 Antibody (D-12) Specifications

Alternate Product Name: GPD2

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 463-492

Epitope Target: GPD2

Epitope Species: human

Product Reactivity/Detection: GPD2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for GPD2 Antibody (D-12): P: Blocking Peptide