golgin 97 Antibody (CDF4): sc-73619

golgin 97 Antibody (CDF4) is a mouse monoclonal IgG1 antibody that detects golgin 97 across multiple species through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Golgin 97 is a crucial component of the trans-Golgi network, functioning as a peripheral membrane protein characterized by an extensive coiled-coil structure, comprising approximately 67% α-helical content, and a C-terminal GRIP domain. This unique structure allows golgin 97 to effectively localize to the cytoplasmic face of the Golgi apparatus, where golgin 97 plays a vital role as a tethering molecule that associates with tubulovesicular carriers during trafficking from the trans-Golgi network to recycling endosomes and early endosomes. The interaction of golgin 97 with the Golgi membrane is facilitated by the G protein family member, Arl1, highlighting golgin 97′s importance in maintaining cellular organization and transport. Additionally, during poxvirus infection, golgin 97 accumulates at viral replication sites, where golgin 97 is incorporated into virions, contributing significantly to virus replication and maturation of both viral membrane and core protein. A rod-like shape, despite lacking a transmembrane domain, allows golgin 97 to protrude from the virion envelope surface, underscoring golgin 97′s multifaceted role in cellular processes and viral pathogenesis.

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golgin 97 Antibody (CDF4) References:

1. Interaction of Arl1-GTP with GRIP domains recruits autoantigens Golgin-97 and Golgin-245/p230 onto the Golgi.  |  Lu, L. and Hong, W. 2003. Mol Biol Cell. 14: 3767-81. PMID: 12972563
2. Autoantigen Golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the trans-golgi network.  |  Lu, L., et al. 2004. Mol Biol Cell. 15: 4426-43. PMID: 15269279
3. E-cadherin transport from the trans-Golgi network in tubulovesicular carriers is selectively regulated by golgin-97.  |  Lock, JG., et al. 2005. Traffic. 6: 1142-56. PMID: 16262725
4. Functional analysis of Arl1 and golgin-97 in endosome-to-TGN transport using recombinant Shiga toxin B fragment.  |  Tai, G., et al. 2005. Methods Enzymol. 404: 442-53. PMID: 16413290
5. A trans-Golgi network resident protein, golgin-97, accumulates in viral factories and incorporates into virions during poxvirus infection.  |  Alzhanova, D. and Hruby, DE. 2006. J Virol. 80: 11520-7. PMID: 16987983
6. A host cell membrane protein, golgin-97, is essential for poxvirus morphogenesis.  |  Alzhanova, D. and Hruby, DE. 2007. Virology. 362: 421-7. PMID: 17276477
7. FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-Golgi network.  |  Jing, J., et al. 2010. Mol Biol Cell. 21: 3041-53. PMID: 20610657
8. Golgi tethering factor golgin-97 suppresses breast cancer cell invasiveness by modulating NF-κB activity.  |  Hsu, RM., et al. 2018. Cell Commun Signal. 16: 19. PMID: 29703230
9. Golgin-97 Targets Ectopically Expressed Inward Rectifying Potassium Channel, Kir2.1, to the trans-Golgi Network in COS-7 Cells.  |  Taneja, TK., et al. 2018. Front Physiol. 9: 1070. PMID: 30123141
10. Targeting the ERK1/2 and p38 MAPK pathways attenuates Golgi tethering factor golgin-97 depletion-induced cancer progression in breast cancer.  |  Liu, YC., et al. 2025. Cell Commun Signal. 23: 22. PMID: 39800687

The preferred golgin 97 antibody is golgin 97 Antibody (CDFX): sc-59820.