GNL2 Antibody (B-8): sc-514050
- GNL2 Antibody (B-8) is a mouse monoclonal IgG2a κ GNL2 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 432-459 within an internal region of GNL2 of human origin
- GNL2 Antibody (B-8) is recommended for detection of GNL2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GNL2 Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GNL2 Antibody (B-8) for WB applications. These reagents are now offered in bundles with GNL2 Antibody (B-8) (see ordering information below).
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GNL2 Antibody (B-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects GNL2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GNL2 Antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GNL2, also known as autoantigen NGP-1, NOG2, or nucleolar GTP-binding protein 2, is a nucleolar guanosine triphosphate-binding protein that plays a vital role in the trafficking of proteins out of the nucleus, a process essential for cellular function and regulation. As a GTPase, GNL2 interacts with the 60S preribosomal subunit within the nucleus, facilitating its export into the cytoplasm, which is crucial for ribosome assembly and protein synthesis. The G domain of GTPases, including GNL2, is responsible for the hydrolysis of GTP, providing the necessary energy for translocating proteins across membranes. GNL2 shares homology and some functional characteristics with nucleostemin (GNL3), another nuclear GTPase, highlighting its importance in ribosomal biogenesis. Notably, GNL2 exhibits the highest expression levels in the testis, underscoring its potential role in reproductive biology and cellular differentiation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GNL2 Antibody (B-8) References:
- Factors affecting nuclear export of the 60S ribosomal subunit in vivo. | Stage-Zimmermann, T., et al. 2000. Mol Biol Cell. 11: 3777-89. PMID: 11071906
- Identification of a 60S preribosomal particle that is closely linked to nuclear export. | Bassler, J., et al. 2001. Mol Cell. 8: 517-29. PMID: 11583615
- Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps. | Saveanu, C., et al. 2001. EMBO J. 20: 6475-84. PMID: 11707418
- The homologous putative GTPases Grn1p from fission yeast and the human GNL3L are required for growth and play a role in processing of nucleolar pre-rRNA. | Du, X., et al. 2006. Mol Biol Cell. 17: 460-74. PMID: 16251348
- Cellular response to 5-fluorouracil (5-FU) in 5-FU-resistant colon cancer cell lines during treatment and recovery. | De Angelis, PM., et al. 2006. Mol Cancer. 5: 20. PMID: 16709241
- A novel lysine-rich domain and GTP binding motifs regulate the nucleolar retention of human guanine nucleotide binding protein, GNL3L. | Rao, MR., et al. 2006. J Mol Biol. 364: 637-54. PMID: 17034816
- GNL3L inhibits activity of estrogen-related receptor gamma by competing for coactivator binding. | Yasumoto, H., et al. 2007. J Cell Sci. 120: 2532-43. PMID: 17623774
- Cloning of a novel nucleolar guanosine 5'-triphosphate binding protein autoantigen from a breast tumor. | Racevskis, J., et al. 1996. Cell Growth Differ. 7: 271-80. PMID: 8822211
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GNL2 Antibody (B-8) | sc-514050 | 200 µg/ml | $322.00 | |||
GNL2 Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-531014 | 200 µg Ab; 10 µg BP | $361.00 | |||
GNL2 Antibody (B-8): m-IgGκ BP-HRP Bundle | sc-524587 | 200 µg Ab, 40 µg BP | $361.00 | |||
GNL2 Antibody (B-8): m-IgG2a BP-HRP Bundle | sc-547661 | 200 µg Ab; 10 µg BP | $361.00 | |||
GNL2 Antibody (B-8) AC | sc-514050 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GNL2 Antibody (B-8) HRP | sc-514050 HRP | 200 µg/ml | $322.00 | |||
GNL2 Antibody (B-8) FITC | sc-514050 FITC | 200 µg/ml | $336.00 | |||
GNL2 Antibody (B-8) PE | sc-514050 PE | 200 µg/ml | $349.00 | |||
GNL2 Antibody (B-8) Alexa Fluor® 488 | sc-514050 AF488 | 200 µg/ml | $364.00 | |||
GNL2 Antibody (B-8) Alexa Fluor® 546 | sc-514050 AF546 | 200 µg/ml | $364.00 | |||
GNL2 Antibody (B-8) Alexa Fluor® 594 | sc-514050 AF594 | 200 µg/ml | $364.00 | |||
GNL2 Antibody (B-8) Alexa Fluor® 647 | sc-514050 AF647 | 200 µg/ml | $364.00 | |||
GNL2 Antibody (B-8) Alexa Fluor® 680 | sc-514050 AF680 | 200 µg/ml | $364.00 | |||
GNL2 Antibody (B-8) Alexa Fluor® 790 | sc-514050 AF790 | 200 µg/ml | $364.00 | |||
GNL2 (B-8) Neutralizing Peptide | sc-514050 P | 100 µg/0.5 ml | $69.00 |