GNL1 Antibody (H-9): sc-514362
- GNL1 Antibody (H-9) is a mouse monoclonal IgG1 κ GNL1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 163-185 within an internal region of GNL1 of human origin
- GNL1 Antibody (H-9) is recommended for detection of GNL1 of human origin by WB, IP, IF and ELISA
- Anti-GNL1 Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GNL1 Antibody (H-9) for WB applications. These reagents are now offered in bundles with GNL1 Antibody (H-9) (see ordering information below).
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GNL1 Antibody (H-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects GNL1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GNL1 (H-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GNL1, or Guanine nucleotide-binding protein-like 1, is a crucial nuclear protein that plays a significant role in regulating the histocompatibility cluster. GNL1 is localized within or near the major histocompatibility complex (MHC) class I region and is a member of the MMR1/HSR1 GTP-binding protein family. The GTPase activity of GNL1 is vital for maintaining normal cell growth, as GNL1 is involved in the circular rearrangement of G-motifs that are essential for ribosome biogenesis. Notably, deletion of GNL1 leads to severe growth defects, characterized by a marked reduction in mature ribosomal RNA species and an accumulation of the 35S pre-rRNA transcript. Furthermore, absence of GNL1 disrupts the export of the ribosomal protein Rpl25a from the nucleolus, resulting in a null phenotype that affects nucleolar structure and function. Upon DNA damage, GNL1 undergoes phosphorylation by kinases such as Atm or ATR, highlighting GNL1′s role in cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GNL1 Antibody (H-9) References:
- The homologous putative GTPases Grn1p from fission yeast and the human GNL3L are required for growth and play a role in processing of nucleolar pre-rRNA. | Du, X., et al. 2006. Mol Biol Cell. 17: 460-74. PMID: 16251348
- A novel lysine-rich domain and GTP binding motifs regulate the nucleolar retention of human guanine nucleotide binding protein, GNL3L. | Rao, MR., et al. 2006. J Mol Biol. 364: 637-54. PMID: 17034816
- [Recent progress in the research on small GTP-binding proteins]. | Tada, M., et al. 2007. Nihon Yakurigaku Zasshi. 130: 373-9. PMID: 18000351
- MgCl2-sensitive and Gpp(NH)p-sensitive antagonist binding states of rat heart muscarinic receptors: preferential detection at ambient temperature assay and location in two subcellular fractions. | Sulakhe, PV., et al. 1990. Mol Cell Biochem. 94: 133-46. PMID: 2374547
- Evolutionary study of multigenic families mapping close to the human MHC class I region. | Vernet, C., et al. 1993. J Mol Evol. 37: 600-12. PMID: 8114113
- Structure and evolution of a member of a new subfamily of GTP-binding proteins mapping to the human MHC class I region. | Vernet, C., et al. 1994. Mamm Genome. 5: 100-5. PMID: 8180467
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GNL1 Antibody (H-9) | sc-514362 | 200 µg/ml | $316.00 | |||
GNL1 Antibody (H-9): m-IgG Fc BP-HRP Bundle | sc-527893 | 200 µg Ab; 10 µg BP | $354.00 | |||
GNL1 Antibody (H-9): m-IgG1 BP-HRP Bundle | sc-533266 | 200 µg Ab; 20 µg BP | $354.00 | |||
GNL1 Antibody (H-9) AC | sc-514362 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GNL1 Antibody (H-9) HRP | sc-514362 HRP | 200 µg/ml | $316.00 | |||
GNL1 Antibody (H-9) FITC | sc-514362 FITC | 200 µg/ml | $330.00 | |||
GNL1 Antibody (H-9) PE | sc-514362 PE | 200 µg/ml | $343.00 | |||
GNL1 Antibody (H-9) Alexa Fluor® 488 | sc-514362 AF488 | 200 µg/ml | $357.00 | |||
GNL1 Antibody (H-9) Alexa Fluor® 546 | sc-514362 AF546 | 200 µg/ml | $357.00 | |||
GNL1 Antibody (H-9) Alexa Fluor® 594 | sc-514362 AF594 | 200 µg/ml | $357.00 | |||
GNL1 Antibody (H-9) Alexa Fluor® 647 | sc-514362 AF647 | 200 µg/ml | $357.00 | |||
GNL1 Antibody (H-9) Alexa Fluor® 680 | sc-514362 AF680 | 200 µg/ml | $357.00 | |||
GNL1 Antibody (H-9) Alexa Fluor® 790 | sc-514362 AF790 | 200 µg/ml | $357.00 | |||
GNL1 (H-9) Neutralizing Peptide | sc-514362 P | 100 µg/0.5 ml | $68.00 |