Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GMF-β CRISPR/Cas9 KO Plasmid (m): sc-425657
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GMF-β CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GMF-β genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GMF-β Antibody (SP-61): sc-134347
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GMF-β CRISPR/Cas9 KO Plasmid (m) | sc-425657 | 20 µg | $397.00 |
Overview
Glia maturation factor beta (GMF-β), encoded by the mouse Gmfb gene, is a conserved cytosolic regulator of actin cytoskeleton remodeling that influences cell shape changes and motility in neural and immune-related contexts. GMF-β interacts with the Arp2/3 complex to modulate actin branch dynamics, linking it to pathways governing neurite outgrowth, synaptic remodeling, and glial cell responses to environmental cues. In the central nervous system, GMF-β has been studied in relation to neuroinflammatory signaling and stress-associated cellular activation programs, where altered cytoskeletal regulation can impact tissue homeostasis. Dysregulated GMF-β–associated processes have relevance to experimental models of neurodegeneration and inflammatory injury through effects on cell migration, reactive gliosis, and cytoskeletal-dependent signaling.
GMF-β CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gmfb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gmfb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gmfb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GMF-β protein expression.
This CRISPR knockout system enables efficient generation of Gmfb-deficient cell models for investigation of GMF-β signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gmfb exon(s) critical for GMF-β function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gmfb genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GMF-β CRISPR/Cas9 KO Plasmid (m) and GMF-β CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gmfb locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GMF-β HDR Plasmid (m) and GMF-β HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gmfb homology arms to support homology-directed repair at defined Gmfb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.