GMAP-210 Antibody (E-2): sc-515208
- GMAP-210 Antibody (E-2) is a mouse monoclonal IgG1 κ GMAP-210 antibody provided at 200 µg/ml
- raised against amino acids 1680-1819 mapping within an internal region of GMAP-210 of human origin
- GMAP-210 Antibody (E-2) is recommended for detection of GMAP-210 of human origin and TRIP11 of mouse and rat origin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GMAP-210 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GMAP-210 Antibody (E-2) for WB applications. These reagents are now offered in bundles with GMAP-210 Antibody (E-2) (see ordering information below).
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GMAP-210 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects GMAP-210 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GMAP-210 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Golgi microtubule-associated protein 210 (GMAP-210), also known as CEV14, Trip11, or Trip230, is a crucial peripheral Golgi protein that localizes to the cis-Golgi network, playing a vital role in maintaining the structure and function of the Golgi apparatus. GMAP-210 is a 1,978 amino acid coiled-coil protein belonging to the golgin family, and GMAP-210′s unique structure allows interaction with microtubule ends, effectively linking the cis-Golgi network to the minus ends of centrosome-nucleated microtubules. This interaction is essential for proper morphology and structural integrity of the Golgi apparatus, as GMAP-210 facilitates Golgi organization in relation to the microtubule cytoskeleton. GMAP-210 has been shown to associate with thyroid hormone receptor-β, indicating potential involvement in signaling pathways. Notably, GMAP-210 overexpression can disrupt the microtubule network, leading to significant enlargement and fragmentation of the Golgi apparatus, while impairing both anterograde and retrograde transport between the endoplasmic reticulum and Golgi apparatus, highlighting GMAP-210′s importance in cellular transport mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GMAP-210 Antibody (E-2) References:
- GMAP-210, A cis-Golgi network-associated protein, is a minus end microtubule-binding protein. | Infante, C., et al. 1999. J Cell Biol. 145: 83-98. PMID: 10189370
- Two splice variants of Golgi-microtubule-associated protein of 210 kDa (GMAP-210) differ in their binding to the cis-Golgi network. | Ramos-Morales, F., et al. 2001. Biochem J. 357: 699-708. PMID: 11463340
- The overexpression of GMAP-210 blocks anterograde and retrograde transport between the ER and the Golgi apparatus. | Pernet-Gallay, K., et al. 2002. Traffic. 3: 822-32. PMID: 12383348
- Positioning the Golgi apparatus. | Linstedt, AD. 2004. Cell. 118: 271-2. PMID: 15294150
- GMAP-210 recruits gamma-tubulin complexes to cis-Golgi membranes and is required for Golgi ribbon formation. | Ríos, RM., et al. 2004. Cell. 118: 323-35. PMID: 15294158
- The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi. | Gillingham, AK., et al. 2004. J Cell Biol. 167: 281-92. PMID: 15504911
- Golgi positioning: are we looking at the right MAP? | Barr, FA. and Egerer, J. 2005. J Cell Biol. 168: 993-8. PMID: 15781478
- Proteomic analysis of vitreous from diabetic macular edema. | Ouchi, M., et al. 2005. Exp Eye Res. 81: 176-82. PMID: 16080911
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GMAP-210 Antibody (E-2) | sc-515208 | 200 µg/ml | $316.00 | |||
GMAP-210 Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-531313 | 200 µg Ab; 10 µg BP | $354.00 | |||
GMAP-210 Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-524972 | 200 µg Ab, 40 µg BP | $354.00 | |||
GMAP-210 Antibody (E-2): m-IgG1 BP-HRP Bundle | sc-544480 | 200 µg Ab; 20 µg BP | $354.00 | |||
GMAP-210 Antibody (E-2) AC | sc-515208 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GMAP-210 Antibody (E-2) HRP | sc-515208 HRP | 200 µg/ml | $316.00 | |||
GMAP-210 Antibody (E-2) FITC | sc-515208 FITC | 200 µg/ml | $330.00 | |||
GMAP-210 Antibody (E-2) PE | sc-515208 PE | 200 µg/ml | $343.00 | |||
GMAP-210 Antibody (E-2) Alexa Fluor® 488 | sc-515208 AF488 | 200 µg/ml | $357.00 | |||
GMAP-210 Antibody (E-2) Alexa Fluor® 546 | sc-515208 AF546 | 200 µg/ml | $357.00 | |||
GMAP-210 Antibody (E-2) Alexa Fluor® 594 | sc-515208 AF594 | 200 µg/ml | $357.00 | |||
GMAP-210 Antibody (E-2) Alexa Fluor® 647 | sc-515208 AF647 | 200 µg/ml | $357.00 | |||
GMAP-210 Antibody (E-2) Alexa Fluor® 680 | sc-515208 AF680 | 200 µg/ml | $357.00 | |||
GMAP-210 Antibody (E-2) Alexa Fluor® 790 | sc-515208 AF790 | 200 µg/ml | $357.00 |