GM2/GD2 Synthase Antibody (C-5): sc-376505

GM2/GD2 Synthase Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects GM2/GD2 Synthase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GM2/GD2 Synthase (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GM2/GD2 synthase protein, encoded by the human gene B4GALNT1, consists of 533 amino acids and plays a crucial role in the biosynthesis of gangliosides, specifically GM2 and GD2, which are sialic acid-containing glyco-sphingolipids involved in important cellular processes such as signal transduction and cell-cell recognition. GM2/GD2 synthase is predominantly expressed in normal brain tissue of vertebrates and features a single hydrophobic segment near its amino-terminus, which is essential for membrane localization and function. GM2/GD2 synthase maintains the balance between simple and complex gangliosides on the cell surface, influencing cellular interactions and signaling pathways. Notably, ganglioside GD2, synthesized by GM2/GD2 synthase, is highly expressed in neuroblastomas and other neuroectoderm-derived tumors, including malignant melanoma and certain gastrointestinal cancers, making GM2/GD2 synthase a valuable marker for neuroblastoma and a potential prognostic indicator for treatment efficacy.

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GM2/GD2 Synthase Antibody (C-5) References:

1. Beta1,4-N-acetylgalactosaminyltransferase--GM2/GD2 synthase: a key enzyme to control the synthesis of brain-enriched complex gangliosides.  |  Furukawa, K., et al. 2002. Biochim Biophys Acta. 1573: 356-62. PMID: 12417418
2. Gangliosides activate the phosphatase activity of the erythrocyte plasma membrane Ca2+-ATPase.  |  Zhang, J., et al. 2005. Arch Biochem Biophys. 444: 1-6. PMID: 16256935
3. Enhanced susceptibility to kainate-induced seizures, neuronal apoptosis, and death in mice lacking gangliotetraose gangliosides: protection with LIGA 20, a membrane-permeant analog of GM1.  |  Wu, G., et al. 2005. J Neurosci. 25: 11014-22. PMID: 16306414
4. Expression of gangliosides on glial and neuronal cells in normal and pathological adult human brain.  |  Marconi, S., et al. 2005. J Neuroimmunol. 170: 115-21. PMID: 16313974
5. Chemoenzymatic synthesis of GM3 and GM2 gangliosides containing a truncated ceramide functionalized for glycoconjugate synthesis and solid phase applications.  |  Jacques, S., et al. 2006. Org Biomol Chem. 4: 142-54. PMID: 16358009
6. Gangliosides enhance migration of mouse B16-melanoma cells through artificial basement membrane alone or in presence of laminin or fibronectin.  |  Saha, S., et al. 2005. Indian J Exp Biol. 43: 1130-8. PMID: 16359123
7. Reversion of the biochemical defects in murine embryonic Sandhoff neurons using a bicistronic lentiviral vector encoding hexosaminidase alpha and beta.  |  Arfi, A., et al. 2006. J Neurochem. 96: 1572-9. PMID: 16441513
8. Endogenous GD3 ganglioside induces apoptosis in U-1242 MG glioma cells.  |  Saqr, HE., et al. 2006. J Neurochem. 96: 1301-14. PMID: 16441517
9. Role of biologically active sphingolipids in tumor growth.  |  Dyatlovitskaya, EV. and Kandyba, AG. 2006. Biochemistry (Mosc). 71: 10-7. PMID: 16457613
10. A randomised phase II trial of a trivalent ganglioside vaccine targeting GM2, GD2 and GD3 combined with immunological adjuvant OPT-821 versus OPT-821 alone in metastatic sarcoma patients rendered disease-free by surgery.  |  Rosenbaum, E., et al. 2022. Eur J Cancer. 176: 155-163. PMID: 36215947
11. Disordered testosterone transport in mice lacking the ganglioside GM2/GD2 synthase gene.  |  Furukawa, K., et al. 2023. FEBS Open Bio. 13: 1615-1624. PMID: 36999634
12. Substrate specificity of beta 1,4-N-acetylgalactosaminyltransferase in vitro and in cDNA-transfected cells. GM2/GD2 synthase efficiently generates asialo-GM2 in certain cells.  |  Yamashiro, S., et al. 1995. J Biol Chem. 270: 6149-55. PMID: 7890749

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GM2/GD2 Synthase Antibody (C-5) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-GM2/GD2 Synthase Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GM2/GD2 Synthase Antibody (C-5) Specifications

Alternate Product Name: GM2/GD2 Synthase

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-180

Epitope Target: GM2

Epitope Species: human

Product Reactivity/Detection: GM2/GD2 synthase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for GM2/GD2 Synthase Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin