GM130 Antibody (H-7): sc-55590

GM130 Antibody (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects GM130 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GM130 (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GM130, a critical cis-Golgi matrix protein, plays a vital role in organizing and maintaining Golgi apparatus function, essential for proper processing and sorting of proteins and lipids within cells. GM130 ′s structure includes a unique amino-terminus that binds to p115 protein, facilitating transport vesicle docking to Golgi membranes, while its carboxy-terminus anchors to the Golgi structure. This dual binding capability maintains Golgi integrity and function, allowing GM130 to participate in vesicle tethering alongside proteins like Giantin. GM130 ′s interactions with p115 and Giantin are essential for transporting materials from endoplasmic reticulum to Golgi, highlighting GM130 ′s importance in cellular trafficking processes. GM130 undergoes post-translational modifications, such as phosphorylation during mitosis, which regulates function and interactions, further underscoring GM130 ′s significance in cellular dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GM130 Antibody (H-7) References:

1. The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo.  |  Seemann, J., et al. 2000. Mol Biol Cell. 11: 635-45. PMID: 10679020
2. Binding relationships of membrane tethering components. The giantin N terminus and the GM130 N terminus compete for binding to the p115 C terminus.  |  Linstedt, AD., et al. 2000. J Biol Chem. 275: 10196-201. PMID: 10744704
3. The mitotic phosphorylation cycle of the cis-Golgi matrix protein GM130.  |  Lowe, M., et al. 2000. J Cell Biol. 149: 341-56. PMID: 10769027
4. The p115-interactive proteins GM130 and giantin participate in endoplasmic reticulum-Golgi traffic.  |  Alvarez, C., et al. 2001. J Biol Chem. 276: 2693-700. PMID: 11035033
5. Determination of functional regions of p125, a novel mammalian Sec23p-interacting protein.  |  Mizoguchi, T., et al. 2000. Biochem Biophys Res Commun. 279: 144-9. PMID: 11112430
6. GM130, a cis-Golgi protein, regulates meiotic spindle assembly and asymmetric division in mouse oocyte.  |  Zhang, CH., et al. 2011. Cell Cycle. 10: 1861-70. PMID: 21552007
7. GM130 is a parallel tetramer with a flexible rod-like structure and N-terminally open (Y-shaped) and closed (I-shaped) conformations.  |  Ishida, R., et al. 2015. FEBS J. 282: 2232-44. PMID: 25787021
8. Loss of GM130 in breast cancer cells and its effects on cell migration, invasion and polarity.  |  Baschieri, F., et al. 2015. Cell Cycle. 14: 1139-47. PMID: 25892554
9. GM130 regulates epithelial-to-mesenchymal transition and invasion of gastric cancer cells via snail.  |  Zhao, J., et al. 2015. Int J Clin Exp Pathol. 8: 10784-91. PMID: 26617790
10. GM130 regulates pulmonary surfactant protein secretion in alveolar type II cells.  |  Pang, Q., et al. 2022. Sci China Life Sci. 65: 193-205. PMID: 33740186
11. The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner.  |  Nakamura, N., et al. 1997. Cell. 89: 445-55. PMID: 9150144
12. Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis.  |  Lowe, M., et al. 1998. Cell. 94: 783-93. PMID: 9753325

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GM130 Antibody (H-7) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-GM130 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GM130 Antibody (H-7) Specifications

Alternate Product Name: GOLGA2

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 191-255

Epitope Target: GM130

Epitope Species: human

Product Reactivity/Detection: GM130

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for GM130 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin