glypican-2 Antibody (F-5): sc-393824
- glypican-2 Antibody (F-5) is a mouse monoclonal IgG1 λ glypican-2 antibody, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 453-468 within an internal region of glypican-2 of human origin
- glypican-2 Antibody (F-5) is recommended for detection of glypican-2 of human origin by WB, IP, IF and ELISA
- Anti-glypican-2 Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for glypican-2 Antibody (F-5) for WB applications. This reagent is now offered in a bundle with glypican-2 Antibody (F-5) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Glypican-2 Antibody (F-5) is a mouse monoclonal IgG1 antibody that detects glypican-2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-glypican-2 antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glypican-2, a member of the glypican family of heparan sulfate proteoglycans, is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor and plays a crucial role in regulating cell adhesion, migration, and proliferation. Glypican-2 is particularly important in the nervous system, where glypican-2 is involved in the motile behaviors of developing neurons. Glypican-2 interacts with midkine (MK), a growth factor that binds to glypican-2′s heparan sulfate chains, promoting cell adhesion and neurite outgrowth. The distinct localization of glypican-2 compared to other glypicans, such as syndecan-3, suggests that these proteins may have different roles in MK-mediated neural functions, highlighting glypican-2′s significance in neural development and signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
glypican-2 Antibody (F-5) References:
- Glypican-3-deficient mice exhibit developmental overgrowth and some of the abnormalities typical of Simpson-Golabi-Behmel syndrome. | Cano-Gauci, DF., et al. 1999. J Cell Biol. 146: 255-64. PMID: 10402475
- Glypican-2 binds to midkine: the role of glypican-2 in neuronal cell adhesion and neurite outgrowth. | Kurosawa, N., et al. 2001. Glycoconj J. 18: 499-507. PMID: 12084985
- Cerebroglycan: an integral membrane heparan sulfate proteoglycan that is unique to the developing nervous system and expressed specifically during neuronal differentiation. | Stipp, CS., et al. 1994. J Cell Biol. 124: 149-60. PMID: 8294498
- Expression of OCI-5/glypican 3 during intestinal morphogenesis: regulation by cell shape in intestinal epithelial cells. | Li, M., et al. 1997. Exp Cell Res. 235: 3-12. PMID: 9281346
- OCI-5/GPC3, a glypican encoded by a gene that is mutated in the Simpson-Golabi-Behmel overgrowth syndrome, induces apoptosis in a cell line-specific manner. | Gonzalez, AD., et al. 1998. J Cell Biol. 141: 1407-14. PMID: 9628896
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
glypican-2 Antibody (F-5) | sc-393824 | 200 µg/ml | $316.00 | |||
glypican-2 Antibody (F-5): m-IgGλ BP-HRP Bundle | sc-524104 | 200 µg Ab, 40 µg BP | $354.00 | |||
glypican-2 Antibody (F-5) AC | sc-393824 AC | 500 µg/ml, 25% agarose | $416.00 | |||
glypican-2 Antibody (F-5) HRP | sc-393824 HRP | 200 µg/ml | $316.00 | |||
glypican-2 Antibody (F-5) FITC | sc-393824 FITC | 200 µg/ml | $330.00 | |||
glypican-2 Antibody (F-5) PE | sc-393824 PE | 200 µg/ml | $343.00 | |||
glypican-2 Antibody (F-5) Alexa Fluor® 488 | sc-393824 AF488 | 200 µg/ml | $357.00 | |||
glypican-2 Antibody (F-5) Alexa Fluor® 546 | sc-393824 AF546 | 200 µg/ml | $357.00 | |||
glypican-2 Antibody (F-5) Alexa Fluor® 594 | sc-393824 AF594 | 200 µg/ml | $357.00 | |||
glypican-2 Antibody (F-5) Alexa Fluor® 647 | sc-393824 AF647 | 200 µg/ml | $357.00 | |||
glypican-2 Antibody (F-5) Alexa Fluor® 680 | sc-393824 AF680 | 200 µg/ml | $357.00 | |||
glypican-2 Antibody (F-5) Alexa Fluor® 790 | sc-393824 AF790 | 200 µg/ml | $357.00 | |||
glypican-2 (F-5) Neutralizing Peptide | sc-393824 P | 100 µg/0.5 ml | $68.00 |